MAB3195

antibody from R&D Systems

Targeting: BSG CD147, EMMPRIN, EMPRIN, OK

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3195

Provider

R&D Systems

Product name

Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Clone TRA-1-85)

Antibody type

Monoclonal

Antigen

2120Ep human embryonal carcinoma cell line

Description

Protein A or G purified from hybridoma culture supernatant

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 µg

References

Submitted references

PDGFA/PDGFRα-regulated GOLM1 promotes human glioma progression through activation of AKT.

Xu R, Ji J, Zhang X, Han M, Zhang C, Xu Y, Wei Y, Wang S, Huang B, Chen A, Zhang D, Zhang Q, Li W, Jiang Z, Wang J, Li X
Journal of experimental & clinical cancer research : CR 2017 Dec 28;36(1):193

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Epigenetic Activation of WNT5A Drives Glioblastoma Stem Cell Differentiation and Invasive Growth.

Hu B, Wang Q, Wang YA, Hua S, Sauvé CG, Ong D, Lan ZD, Chang Q, Ho YW, Monasterio MM, Lu X, Zhong Y, Zhang J, Deng P, Tan Z, Wang G, Liao WT, Corley LJ, Yan H, Zhang J, You Y, Liu N, Cai L, Finocchiaro G, Phillips JJ, Berger MS, Spring DJ, Hu J, Sulman EP, Fuller GN, Chin L, Verhaak RGW, DePinho RA
Cell 2016 Nov 17;167(5):1281-1295.e18

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Bacterially expressed full-length recombinant Plasmodium falciparum RH5 protein binds erythrocytes and elicits potent strain-transcending parasite-neutralizing antibodies.

Reddy KS, Pandey AK, Singh H, Sahar T, Emmanuel A, Chitnis CE, Chauhan VS, Gaur D
Infection and immunity 2014 Jan;82(1):152-64

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Neutralization of Plasmodium falciparum merozoites by antibodies against PfRH5.

Douglas AD, Williams AR, Knuepfer E, Illingworth JJ, Furze JM, Crosnier C, Choudhary P, Bustamante LY, Zakutansky SE, Awuah DK, Alanine DG, Theron M, Worth A, Shimkets R, Rayner JC, Holder AA, Wright GJ, Draper SJ
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 1;192(1):245-58

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Direct in vivo evidence for tumor propagation by glioblastoma cancer stem cells.

Lathia JD, Gallagher J, Myers JT, Li M, Vasanji A, McLendon RE, Hjelmeland AB, Huang AY, Rich JN
PloS one 2011;6(9):e24807

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human TRA-1-85/CD147 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue, human heart tissue, and human liver tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TRA-1-85/CD147 at approximately 40-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Western Blot Shows Human TRA-1-85/CD147 Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and TRA-1-85/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TRA-1-85/CD147 at approximately 51 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

TRA-1-85/CD147 in BG01V Human Embryonic Stem Cells. TRA-1-85/CD147 was detected in immersion fixed BG01V human embryonic stem cells on irradiated mouse embryonic fibroblasts using Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of TRA-1-85/CD147 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes and monocytes were stained with either (A) Mouse Anti-Human TRA-1-85/CD147 Monoclonal Antibody (Catalog # MAB3195) or (B) Mouse IgG₁ Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).