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- References [1]
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- Western blot [2]
- Flow cytometry [1]
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- Product number
- NB100-2443 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2443, RRID:AB_10002160
- Product name
- Goat Polyclonal GATA-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Developmental stage-selective effect of somatically mutated leukemogenic transcription factor GATA1.
Li Z, Godinho FJ, Klusmann JH, Garriga-Canut M, Yu C, Orkin SH
Nature genetics 2005 Jun;37(6):613-9
Nature genetics 2005 Jun;37(6):613-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GATA-1 Antibody [NB100-2443] - (0.3ug/ml) staining of human PBMC lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GATA-1 Antibody [NB100-2443] - Staining of K562 nuclear cell lysate (A) and negative control Human Hippocampus (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GATA-1 Antibody [NB100-2443] - Analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
