328802

antibody from BioLegend

Targeting: SELPLG CD162, PSGL-1

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

328802

Provider

BioLegend

Proper citation

BioLegend Cat#328802, RRID:AB_961085

Product name

Purified anti-human CD162

Antibody type

Monoclonal

Antigen

PSGL-1 transfected murine 300.19 pre B-cell line

Description

CD162, also known as p-selectin glycoprotein ligand-1 (PSGL-1), is a 120 - 220 kD, mucin-like type I transmembrane glycoprotein. CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin). The interactions between P-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) mediate the earliest "rolling" of leukocytes on the lumenal surface of activated endothelium, and the interaction between leukocytes and activated platelets or other leukocytes found at sites of inflammation. CD162 is expressed on neutrophils, monocytes, and most lymphocytes including NK and T cells but PSGL-1 stains B cells at significantly lower levels than other cell types.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4° C.

References

Submitted references

Demyelinating diseases: myeloperoxidase as an imaging biomarker and therapeutic target.

Forghani R, Wojtkiewicz GR, Zhang Y, Seeburg D, Bautz BR, Pulli B, Milewski AR, Atkinson WL, Iwamoto Y, Zhang ER, Etzrodt M, Rodriguez E, Robbins CS, Swirski FK, Weissleder R, Chen JW
Radiology 2012 May;263(2):451-60

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Adaptive mutations in the genomes of enterovirus 71 strains following infection of mouse cells expressing human P-selectin glycoprotein ligand-1.

Miyamura K, Nishimura Y, Abo M, Wakita T, Shimizu H
The Journal of general virology 2011 Feb;92(Pt 2):287-91

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood lymphocytes stained with purified KPL-1, followed by anti-mouse IgG FITC

Conjugate

Unconjugated