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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00005551-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005551-D01, RRID:AB_10658343
- Product name
- PRF1 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human PRF1 protein.
- Antigen sequence
MAARLLLLGILLLLLPLPVPAPCHTAARSECKRSH
KFVPGAWLAGEGVDVTSLRRSGSFPVDTQRFLRPD
GTCTLCENALQEGTLQRLPLALTNWRAQGSGCQRH
VTRAKVSSTEAVARDAARSIRNDWKVGLDVTPKPT
SNVHVSVAGSHSQAANFAAQKTHQDQYSFSTDTVE
CRFYSFHVVHTPPLHPDFKRALGDLPHHFNASTQP
AYLRLISNYGTHFIRAVELGGRISALTALRTCELA
LEGLTDNEVEDCLTVEAQVNIGIHGSISAEAKACE
EKKKKHKMTASFHQTYRERHSEVVGGHHTSINDLL
FGIQAGPEQYSAWVNSLPGSPGLVDYTLEPLHVLL
DSQDPRREALRRALSQYLTDRARWRDCSRPCPPGR
QKSPRDPCQCVCHGSAVTTQDCCPRQRGLAQLEVT
FIQAWGLWGDWFTATDAYVKLFFGGQELRTSTVWD
NNNPIWSVRLDFGDVLLATGGPLRLQVWDQDSGRD
DDLLGTCDQAPKSGSHEVRCNLNHGHLKFRYHARC
LPHLGGGTCLDYVPQMLLGEPPGNRSGAVW- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PRF1 expression in transfected 293T cell line (H00005551-T02) by PRF1 MaxPab polyclonal antibody.Lane 1: PRF1 transfected lysate(61.40 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PRF1 transfected lysate using anti-PRF1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PRF1 purified MaxPab mouse polyclonal antibody (B01P) (H00005551-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
