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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [3]
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- Product number
- NB300-137 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-137, RRID:AB_10001590
- Product name
- Rabbit Polyclonal Peripherin Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. Specifically recognizes peripherin, showing no cross reactivity with other Class III intermediate filament proteins.
- Reactivity
- Human, Mouse, Rat, Bovine, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Murine neuroblastoma cell lines developed by conditional reprogramming preserve heterogeneous phenotypes observed in vivo.
Collagen VI regulates peripheral nerve myelination and function.
Progesterone withdrawal promotes remodeling processes in the nonpregnant mouse cervix.
Motor axon guidance of the mammalian trochlear and phrenic nerves: dependence on the netrin receptor Unc5c and modifier loci.
Increased innervation and ripening of the prepartum murine cervix.
Viral delivery of NR2D subunits reduces Mg2+ block of NMDA receptor and restores NT-3-induced potentiation of AMPA-kainate responses in maturing rat motoneurons.
Krawczyk E, Hong SH, Galli S, Trinh E, Wietlisbach L, Misiukiewicz SF, Tilan JU, Chen YS, Schlegel R, Kitlinska J
Laboratory investigation; a journal of technical methods and pathology 2020 Jan;100(1):38-51
Laboratory investigation; a journal of technical methods and pathology 2020 Jan;100(1):38-51
Collagen VI regulates peripheral nerve myelination and function.
Chen P, Cescon M, Megighian A, Bonaldo P
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Mar;28(3):1145-56
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2014 Mar;28(3):1145-56
Progesterone withdrawal promotes remodeling processes in the nonpregnant mouse cervix.
Yellon SM, Burns AE, See JL, Lechuga TJ, Kirby MA
Biology of reproduction 2009 Jul;81(1):1-6
Biology of reproduction 2009 Jul;81(1):1-6
Motor axon guidance of the mammalian trochlear and phrenic nerves: dependence on the netrin receptor Unc5c and modifier loci.
Burgess RW, Jucius TJ, Ackerman SL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 May 24;26(21):5756-66
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 May 24;26(21):5756-66
Increased innervation and ripening of the prepartum murine cervix.
Kirby LS, Kirby MA, Warren JW, Tran LT, Yellon SM
Journal of the Society for Gynecologic Investigation 2005 Dec;12(8):578-85
Journal of the Society for Gynecologic Investigation 2005 Dec;12(8):578-85
Viral delivery of NR2D subunits reduces Mg2+ block of NMDA receptor and restores NT-3-induced potentiation of AMPA-kainate responses in maturing rat motoneurons.
Arvanian VL, Bowers WJ, Petruska JC, Motin V, Manuzon H, Narrow WC, Federoff HJ, Mendell LM
Journal of neurophysiology 2004 Oct;92(4):2394-404
Journal of neurophysiology 2004 Oct;92(4):2394-404
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Peripherin Antibody [NB300-137] Detection of the 57 kDa peripherin protein in whole rat brain stem homogenate using NB 300-137 (1:20,000). The faint 48 kDa band is a peripherin molecule.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Peripherin Antibody [NB300-137] - Analysis of whole rat spinal cord lysate stained with NB300-137, at dilution of 1:1,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~57 kDa corresponds to Peripherin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Peripherin Antibody [NB300-137] - Analysis of tissue and cell lysates using Peripherin antibody, dilution 1:10000 in green: [1] protein standard, [2] rat spinal cord, [3] mouse spinal cord, [4] pig spinal cord, [5] cow spinal cord, [6] SH-SY5Y cells and [7] PC12 cells. The major band at ~57kDa corresponds to the major peripherin protein isoform, while other bands presumably represent protein products of alternate transcripts of the peripherin gene.
