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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [2]
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- Product number
- ALX-210-732-R100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-210-732-R100, RRID:AB_2052724
- Product name
- TRAIL (human) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 μl
- Storage
- +4°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Tumor-associated B7-H1 promotes T-cell apoptosis: a potential mechanism of immune evasion.
Hypericin photo-induced apoptosis involves the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and activation of caspase-8.
Dong H, Strome SE, Salomao DR, Tamura H, Hirano F, Flies DB, Roche PC, Lu J, Zhu G, Tamada K, Lennon VA, Celis E, Chen L
Nature medicine 2002 Aug;8(8):793-800
Nature medicine 2002 Aug;8(8):793-800
Hypericin photo-induced apoptosis involves the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and activation of caspase-8.
Schempp CM, Simon-Haarhaus B, Termeer CC, Simon JC
FEBS letters 2001 Mar 23;493(1):26-30
FEBS letters 2001 Mar 23;493(1):26-30
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunostaining of HEK 293T cells transfected with a expression plasmid coding for human TRAIL (left panels), or mock transfected (right panel). Method: 3 days after transfection of cells as indicated, cells were fixed with 4% formaldehyde or acetone for 5 min. at RT. Slides were blocked with IgG, and incubated for 1 hour with PAb to TRAIL (Prod. No. ALX-210-732) at a dilution ofÊ1:100 or control rabbit IgG in PBS containing 1% BSA. After washes with PBS, samples were incubated with a biotinylated secondary antibody to rabbit IgG for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Detection of recombinant human TRAIL (Prod. No. ALX-522-003Ê) but not of recombinant human FasL (Prod. No. ALX-522-001). Method: Recombinant soluble TRAIL, and recombinant soluble FasL as negative control, were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and detected with PAb to TRAIL (human) (Prod. No. ALX-210-732)Êat a dilution of 1:500. Proteins were visualized using a peroxidase-conjugated antibody to rabbit IgG and a chemiluminescence detection system.
