14151-1-AP

antibody from Invitrogen Antibodies

Targeting: RP2 NM23-H10, NME10, TBCCD2

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

14151-1-AP

Provider

Invitrogen Antibodies

Product name

RP2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MGCFFSKRR KADKESRPEN EEERPKQYSW DQREKVDPKD YMFSGLKDET VGRLPGTVAG QQFLIQDCEN CNIYIFDHSA TVTIDDCTNC IIFLGPVKGS VFFRNCRDCK CTLACQQFRV RDCRKLEVFL CCATQPIIES SSNIKFGCFQ WYYPELAFQF KDAGLSIFNN TWSNIHDFTP VSGELNWSLL PEDAVVQDYV PIPTTEELKA VRVSTEANRS IVPISRGQRQ KSSDESCLVV LFAGDYTIAN ARKLIDEMVG KGFFLVQTKE VSMKAEDAQR VFREKAPDFL PLLNKGPVIA LEFNGDGAVE VCQLIVNEIF NGTKMFVSES KETASGDVDS FYNFADIQMG I (1-350 aa encoded by BC043348)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.21 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 14151-1-AP (RP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Y79 cells were subjected to SDS PAGE followed by western blot with 14151-1-AP (RP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14151-1-AP (RP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 14151-1-AP (RP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 14151-1-AP (RP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer using 14151-1-AP (RP2 antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer using 14151-1-AP (RP2 antibody) at dilution of 1:50 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug RP2 antibody (14151-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RP2 (IP: 14151-1-AP, 4 µg; Detection: 14151-1-AP 1:500) with Y79 cells lysate 2000 µg.