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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3149 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3149, RRID:AB_1572374
- Product name
- CD9 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CD9.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The transferrin receptor and the tetraspanin web molecules CD9, CD81, and CD9P-1 are differentially sorted into exosomes after TPA treatment of K562 cells.
A novel cysteine cross-linking method reveals a direct association between claudin-1 and tetraspanin CD9.
The tetraspanin CD9 inhibits the proliferation and tumorigenicity of human colon carcinoma cells.
Abache T, Le Naour F, Planchon S, Harper F, Boucheix C, Rubinstein E
Journal of cellular biochemistry 2007 Oct 15;102(3):650-64
Journal of cellular biochemistry 2007 Oct 15;102(3):650-64
A novel cysteine cross-linking method reveals a direct association between claudin-1 and tetraspanin CD9.
Kovalenko OV, Yang XH, Hemler ME
Molecular & cellular proteomics : MCP 2007 Nov;6(11):1855-67
Molecular & cellular proteomics : MCP 2007 Nov;6(11):1855-67
The tetraspanin CD9 inhibits the proliferation and tumorigenicity of human colon carcinoma cells.
Ovalle S, Gutiérrez-López MD, Olmo N, Turnay J, Lizarbe MA, Majano P, Molina-Jiménez F, López-Cabrera M, Yáñez-Mó M, Sánchez-Madrid F, Cabañas C
International journal of cancer 2007 Nov 15;121(10):2140-52
International journal of cancer 2007 Nov 15;121(10):2140-52
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CD9 polyclonal antibody (Cat # PAB3149) in HepG2 cell line lysates (35 ug/lane). CD9 (arrow) was detected using the purified polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CD9 (arrow) using rabbit CD9 polyclonal antibody (Cat # PAB3149). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD9 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of CD9 polyclonal antibody (Cat # PAB3149) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using CD9 polyclonal antibody (Cat # PAB3149)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
