Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- Ab180142 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- CD9 Mouse mAb (Biotin)
- Antibody type
- Monoclonal
- Description
- Application:Flow: ²0.5_g/testFor flow cytometric staining, the suggested use of this reagent is ²0.5 _g per million cells in 100 µL staining volume or 100 µL of whole blood.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Antibody clone number
- HI9a
- Vial size
- 100_g,10_g,25_g
- Concentration
- 0,5 mg/ml
- Storage
- The antibody solution should be stored undiluted between 2¡C and 8¡C, and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- Aladdin Scientific (provider)
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- Experimental details
- CD9 Mouse mAb (Biotin) (Ab180142) - Flow Cytometry Flow cytometry analysis of CD9 expression by MCF-7 cells (red) compared to an isotype control (blue). Cells were stained with CD9 Mouse mAb (Biotin) (Ab180142) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.