AMAb90905

antibody from Atlas Antibodies

Targeting: CD40 Bp50, p50, TNFRSF5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

AMAb90905

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb90905, RRID:AB_2665719

Product name

Anti-CD40

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

KQYLINSQCCSLCQPGQKLVSDCTEFTETECLPCG
ESEFLDTWNRETHCHQHKYCDPNLGLRVQQKGTSE
TDTICTCE


Isotype

IgG

Antibody clone number

CL1673

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

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Experimental details

Lane 1: Marker [kDa]Lane 2: Human spleen

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

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Experimental details

Immunohistochemistry analysis in human tonsil and cerebral cortex tissues using AMAb90905 antibody. Corresponding CD40 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows strong membranous immunoreactivity in the reaction center cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows membranous positivity in a subset of lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

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Experimental details

Immunohistochemical staining of human duodenum shows moderate immunoreactivity in a subset of lymphoid cells in lamina propria.

Supportive validation

Submitted by

Atlas Antibodies (provider)

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Experimental details

Immunohistochemical staining of human endometrium shows positivity in a subset of lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

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Experimental details

Immunohistochemical staining of human renal cancer (clear cell carcinoma) shows membranous positivity in tumor cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

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Experimental details

Immunohistochemical staining of human cerebellum shows absence of immunoreactivity (negative control).

Supportive validation

Submitted by

Atlas Antibodies (provider)

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Experimental details

Immunohistochemical staining of human tonsil shows strong membranous positivity in germinal center cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon shows moderate membranous positivity in lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows no positivity in neuronal cells as expected.