A700-056

antibody from Invitrogen Antibodies

Targeting: CD40 Bp50, p50, TNFRSF5

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A700-056

Provider

Invitrogen Antibodies

Product name

CD40 Recombinant Rabbit Monoclonal Antibody (BLR056F)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

BLR056F

Vial size

100 µL

Concentration

250 µg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD40 by western blot. Samples: Whole cell lysate (50 µg) from Raji, HEK293T, U2OS, GaMG, Daudi, A-549, Ramos, HDLM-2, RKO, Jurkat, and MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody [BLR056F] (Product # A700-056 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-RPS3 (Product # A303-840A).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD40 by immunocytochemistry.Sample: FFPE section of Raji cells.Antibody: Rabbit anti-CD40 recombinant monoclonal antibody [BLR056F] (Product # A700-056; lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD40 by immunohistochemistry. AntibodySample: FFPE section of human breast carcinoma.Antibody: Rabbit anti-CD40 recombinant monoclonal antibody (BLR056F) (A700-056 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD40 by immunohistochemistry.Sample: FFPE section of human tonsil.Antibody: Rabbit anti-CD40 recombinant monoclonal antibody [BLR056F] (Product # A700-056 lot 1) used at 1:250.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD40 (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD40 recombinant monoclonal antibody [BLR056F] (Product # A700-056 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: Opal™. Counterstain: DAPI (blue).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human CD40 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Raji cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD40 recombinant monoclonal antibody [BLR056F] (Product # A700-056 lot 1) used for IP at 5 µL per reaction. CD40 was also immunoprecipitated by rabbit anti-CD40 recombinant monoclonal antibody (BL-790-1G12). For blotting immunoprecipitated CD40 (Product # A700-056) was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.