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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
- Other assay [1]
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- Product number
- AF632 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD40/TNFRSF5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CD40 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse CD40 and recombinant rat CD40 is observed, and less 1% cross-reactivity with recombinant human (rh) TNF RI, rhsTNF RII, rhCD40 Ligand, rh4-1BB, rhCD27, rhCD30, rhDR3, rhDR6, rhFas, rhGITR, rhHVEM, rhNGF R, rhRANK and rhRELT is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P25942- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Expression, purification and biological characterization of the extracellular domain of CD40 from Pichia pastoris.
CD19 and CD32b differentially regulate human B cell responsiveness.
CD40.FasL inhibits human T cells: evidence for an auto-inhibitory loop-back mechanism.
Disruption of CD40/CD40 ligand interaction with cleavage of CD40 on human gingival fibroblasts by human leukocyte elastase resulting in down-regulation of chemokine production.
Zhan Y, Wei Y, Chen P, Zhang H, Liu D, Zhang J, Liu R, Chen R, Zhang J, Mo W, Zhang X
BMC biotechnology 2016 Jan 25;16:8
BMC biotechnology 2016 Jan 25;16:8
CD19 and CD32b differentially regulate human B cell responsiveness.
Karnell JL, Dimasi N, Karnell FG 3rd, Fleming R, Kuta E, Wilson M, Wu H, Gao C, Herbst R, Ettinger R
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1480-90
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 15;192(4):1480-90
CD40.FasL inhibits human T cells: evidence for an auto-inhibitory loop-back mechanism.
Dranitzki-Elhalel M, Huang JH, Sasson M, Rachmilewitz J, Parnas M, Tykocinski ML
International immunology 2007 Apr;19(4):355-63
International immunology 2007 Apr;19(4):355-63
Disruption of CD40/CD40 ligand interaction with cleavage of CD40 on human gingival fibroblasts by human leukocyte elastase resulting in down-regulation of chemokine production.
Nemoto E, Tada H, Shimauchi H
Journal of leukocyte biology 2002 Sep;72(3):538-45
Journal of leukocyte biology 2002 Sep;72(3):538-45
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CD40/TNFRSF5 by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human CD40/TNFRSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF632) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD40/TNFRSF5 at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Human CD40/TNFRSF5 Antibody Stimulates Cell Proliferation in Human B Cells. Goat Anti-Human CD40/TNFRSF5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF632) stimulates human B cell proliferation in the presence of Recombinant Human IL-4 (Catalog # 204-IL) in a dose-dependent manner, as measured by Resazurin (Catalog # AR002). The ED50 for this effect is typically 3-12 ng/mL
