GTX628902

antibody from GeneTex

Targeting: ICAM3 CD50, CDW50, ICAM-R

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX628902

Provider

GeneTex

Product name

CD50 antibody [EPR3994]

Antibody type

Monoclonal

Host

Rabbit

Vial size

100 µl

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HA tag antibody [GT4810] detects HA tag protein by Western blot analysis.A. 30 ?g HeLa whole cell lysate/extractB. 30 ?g whole cell lysate/extract of HA tagged protein expressing HeLa cells5 % SDS-PAGEHA tag antibody [GT4810] (GTX628902) dilution: 1:5000

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HA tag antibody [GT4810] detects HA-recombinant protein by immunofluorescent analysis. Sample: HA tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: HA-recombinant protein stained by HA tag antibody [GT4810] (GTX628902) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HA tag antibody [GT4810] immunoprecipitates HA tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 £gg whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 £gg of pre-immune mouse IgG C : Immunoprecipitation of HA tagged protein by 2.5 £gg of HA tagg antibody (GTX628902) 10% SDS-PAGE The immunoprecipitated HA tagged protein was detected by HA tag antibody [GT4810] (GTX628902) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.