AM33033PU-N

antibody from Acris Antibodies GmbH

Targeting: ICAM3 CD50, CDW50, ICAM-R

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM33033PU-N

Provider

Acris Antibodies GmbH

Product name

anti CD50 / ICAM3

Antibody type

Monoclonal

Antigen

Derived by fusion of mouse Ag8.653 cells with spleen cells from a BALB/c mouse immunized with an ICAM-3/HEK transfectant.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

MA4

Vial size

0.1 mg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Purified ICAM-3-Fc was separated by using PAGE and transferred via electroblotting to nitrocellulose membrane. The membrane was probed with MA4 at a dilution of 1/200 (using TBST – TBS+0.05% tween 20) which equates to a concentration of 5 µg/ml and mAb binding detected by anti-mouse-HRP (1/1000). This single band on western blot analysis is consistent with the published reports using this recombinant ICAM-3-Fc which contained only domains 1 and 2 of ICAM-3 fused to human Fc. The band shown is approximately 80kDa.

ELISA

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Purified ICAM-3-Fc or CD14-Fc was captured to an ELISA plate and probed with the indicated dilution of mAb MA4. The binding of mAb was detected anti-Mouse-HRP (1/1000) and OPD detection system. Data shown are mean ± SD of a representative experiment. MA4 shows clear specificity for ICAM-3.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Mutu (human B cell line) that were wild type (left panel) or ICAM-3-deficient (right panel) were stained with mAb MA4 (blue line) or an isotype control (solid red). Per tube, 2 x 105 cells were stained with 75 µl of mAb at the indicated concentrations. Following 30 min incubation at 4°C, unbound Ab was removed by washing and bound mAb detected by staining with goat anti-mouse-PE for 30 min at 4°C. Flow cytometric histograms of 5000 events per sample are shown.