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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Flow cytometry [1]
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- Product number
- AM33033PU-N - Provider product page
- Provider
- OriGene
- Product name
- ICAM3 mouse monoclonal antibody, clone MA4, Purified
- Antibody type
- Monoclonal
- Description
- ICAM3 mouse monoclonal antibody, clone MA4, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ICAM3
- Isotype
- IgG
- Antibody clone number
- MA4
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Purified ICAM-3-Fc was separated by using PAGE and transferred via electroblotting to nitrocellulose membrane. The membrane was probed with MA4 at a dilution of 1/200 (using TBST - TBS+0.05% tween 20) which equates to a concentration of 5 ug/ml and mAb binding detected by anti-mouse-HRP (1/1000). This single band on western blot analysis is consistent with the published reports using this recombinant ICAM-3-Fc which contained only domains 1 and 2 of ICAM-3 fused to human Fc. The band shown is approximately 80kDa.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Purified ICAM-3-Fc or CD14-Fc was captured to an ELISA plate and probed with the indicated dilution of mAb MA4. The binding of mAb was detected anti-Mouse-HRP (1/1000) and OPD detection system. Data shown are mean ¡À SD of a representative experiment. MA4 shows clear specificity for ICAM-3.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Mutu (human B cell line) that were wild type (left panel) or ICAM-3-deficient (right panel) were stained with mAb MA4 (blue line) or an isotype control (solid red). Per tube, 2 x 105 cells were stained with 75 ?l of mAb at the indicated concentrations. Following 30 min incubation at 4°C, unbound Ab was removed by washing and bound mAb detected by staining with goat anti-mouse-PE for 30 min at 4°C. Flow cytometric histograms of 5000 events per sample are shown.
- Validation comment
- FC
