Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- AM33036PU-N - Provider product page
- Provider
- OriGene
- Product name
- ICAM3 mouse monoclonal antibody, clone BH10, Purified
- Antibody type
- Monoclonal
- Description
- ICAM3 mouse monoclonal antibody, clone BH10, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ICAM3
- Isotype
- IgG
- Antibody clone number
- BH10
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
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- OriGene (provider)
- Main image
- Experimental details
- Jurkat T cells that expressed high (ICAM-3 high= red) or low (ICAM-3 low= blue) levels of ICAM-3 were stained with mAb BH10. 5 x 105 cells were stained with 100ul of mAb. Following 30 min incubation at 4°C, unbound Ab was removed by washing and bound mAb detected by staining with goat antimouse- FITC for 30 min at 4°C. Flow cytometric histogram of 5000 events per sample is shown.
- Validation comment
- FC