17699-1-AP

antibody from Invitrogen Antibodies

Targeting: ICAM3 CD50, CDW50, ICAM-R

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

17699-1-AP

Provider

Invitrogen Antibodies

Product name

ICAM3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GLGLFVNTS APRQLRTFVL PVTPPRLVAP RFLEVETSWP VDCTLDGLFP ASEAQVYLAL GDQMLNATVM NHGDTLTATA TATARADQEG AREIVCNVTL GGERREAREN LTVFSFLGPI VNLSEPTAHE GSTVTVSCMA GARVQVTLDG VPAAAPGQPA QLQLNATESD DGRSFFCSAT LEVDGEFLHR NSSVQLRVLY GPKIDRATCP QHLKWKDKTR HVLQCQARGN PYPELRCLKE GSSREVPVGI PFFVNVTHNG TYQCQASSSR GKYTLVVVMD IEAGSSHFVP VFVAVLLTLG VVTIVLALMY VFREHQRSGS YHVREESTYL PLTSMQPTEA MGEEPSRAE (200-547 aa encoded by BC058903)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.19 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

U-937 cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Ramos cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 Jurkat cells were stained with 0.5ug ICAM3 antibody (17699-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ICAM3 (IP:17699-1-AP, 4ug; Detection:17699-1-AP 1:500) with Jurkat cells lysate 4000 ug.