MA5-12008

antibody from Invitrogen Antibodies

Targeting: B3GAT1 CD57, GlcAT-P, HNK-1, LEU7, NK-1

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-12008

Provider

Invitrogen Antibodies

Product name

CD57 Monoclonal Antibody (NK1)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-12008 targets CD57 in FACS and IHC (P) applications and shows reactivity with Human samples.

Antibody clone number

NK1

Concentration

0.2 mg/mL

References

Submitted references

Association of decreased expression of the macrophage scavenger receptor MARCO with tumor progression and poor prognosis in human hepatocellular carcinoma.

Sun H, Song J, Weng C, Xu J, Huang M, Huang Q, Sun R, Xiao W, Sun C
Journal of gastroenterology and hepatology 2017 May;32(5):1107-1114

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Stromal expression of Fer suppresses tumor progression in renal cell carcinoma and is a predictor of survival.

Mitsunari K, Miyata Y, Watanabe SI, Asai A, Yasuda T, Kanda S, Sakai H
Oncology letters 2017 Feb;13(2):834-840

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CD200R, a co-inhibitory receptor on immune cells, predicts the prognosis of human hepatocellular carcinoma.

Sun H, Xu J, Huang M, Huang Q, Sun R, Xiao W, Sun C
Immunology letters 2016 Oct;178:105-13

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CD8(+) T Cells from Human Neonates Are Biased toward an Innate Immune Response.

Galindo-Albarrán AO, López-Portales OH, Gutiérrez-Reyna DY, Rodríguez-Jorge O, Sánchez-Villanueva JA, Ramírez-Pliego O, Bergon A, Loriod B, Holota H, Imbert J, Hernández-Mendoza A, Ferrier P, Carrillo-de Santa Pau E, Valencia A, Spicuglia S, Santana MA
Cell reports 2016 Nov 15;17(8):2151-2160

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Retrospective analysis of clinicopathological features of solid pseudopapillary neoplasm of the pancreas.

Ugras N, Yerci Ö, Coşkun SK, Ocakoğlu G, Sarkut P, Dündar HZ
The Kaohsiung journal of medical sciences 2016 Jul;32(7):356-61

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Aberrant immunoarchitecture distinguishes hyperplastic germinal centres in pattern 1 angioimmunoblastic T-cell lymphoma from reactive follicles.

Tan LH, Tan SY
Hematological oncology 2014 Sep;32(3):145-54

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NKT-associated hedgehog and osteopontin drive fibrogenesis in non-alcoholic fatty liver disease.

Syn WK, Agboola KM, Swiderska M, Michelotti GA, Liaskou E, Pang H, Xie G, Philips G, Chan IS, Karaca GF, Pereira Tde A, Chen Y, Mi Z, Kuo PC, Choi SS, Guy CD, Abdelmalek MF, Diehl AM
Gut 2012 Sep;61(9):1323-9

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Different morphology, stage and treatment affect immune cell infiltration and long-term outcome in patients with non-small-cell lung carcinoma.

da Costa Souza P, Parra ER, Atanazio MJ, da Silva OB, Noleto GS, Ab'Saber AM, de Morais Fernezlian S, Takagaki T, Capelozzi VL
Histopathology 2012 Oct;61(4):587-96

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Epithelioid angiosarcoma arising in schwannoma: report of three Chinese cases with review of the literature.

Li C, Chen Y, Zhang H, Zheng X, Wang J
Pathology international 2012 Jul;62(7):500-5

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Tumour eosinophilia combined with an immunohistochemistry panel is useful in the differentiation of type B3 thymoma from thymic carcinoma.

Khoury T, Chandrasekhar R, Wilding G, Tan D, Cheney RT
International journal of experimental pathology 2011 Apr;92(2):87-96

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Characterization of cytotoxic immune response in skin and mucosal lesions of paracoccidioidomycosis.

Pagliari C, Pereira NV, Kanashiro L, Stegun FW, Croda J, Duarte MI, Sotto MN
Journal of cutaneous pathology 2010 May;37(5):565-70

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Biomolecular network reconstruction identifies T-cell homing factors associated with survival in colorectal cancer.

Mlecnik B, Tosolini M, Charoentong P, Kirilovsky A, Bindea G, Berger A, Camus M, Gillard M, Bruneval P, Fridman WH, Pagès F, Trajanoski Z, Galon J
Gastroenterology 2010 Apr;138(4):1429-40

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Are CD57+ natural killer cells really important in early pregnancy failure?

Ozcimen EE, Kiyici H, Uckuyu A, Yanik FF
Archives of gynecology and obstetrics 2009 Apr;279(4):493-7

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Natural killer cells and alterations in collagen density: signs of periradicular herpesvirus infection?

Saboia-Dantas CJ, Coutrin de Toledo LF, Siqueira JF Jr, Sampaio-Filho HR, Carvalho JJ, Pereira MJ
Clinical oral investigations 2008 Jun;12(2):129-35

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Isolation of neural crest-derived stem cells from rat embryonic mandibular processes.

Zhang J, Duan X, Zhang H, Deng Z, Zhou Z, Wen N, Smith AJ, Zhao W, Jin Y
Biology of the cell 2006 Oct;98(10):567-75

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Primary cutaneous histiocyte and neutrophil-rich CD30+ and CD56+ anaplastic large-cell lymphoma with prominent angioinvasion and nerve involvement in the forehead and scalp of an immunocompetent woman.

Boudova L, Kazakov DV, Jindra P, Sima R, Vanecek T, Kuntscher V, Vera V, Bouda J, Michal M
Journal of cutaneous pathology 2006 Aug;33(8):584-9

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Pigmented solid-pseudopapillary neoplasm of the pancreas.

Daum O, Sima R, Mukensnabl P, Vanecek T, Brouckova M, Benes Z, Michal M
Pathology international 2005 May;55(5):280-4

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Pulmonary large cell carcinoma with rhabdoid phenotype.

Yilmazbayhan D, Ates LE, Dilege S, Gulluoglu M, Tanju S, Kalayci G
Annals of diagnostic pathology 2005 Aug;9(4):223-6

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Lipomatous hemangiopericytoma of the head and neck: immunohistochemical and DNA ploidy analyses.

Alrawi SJ, Deeb G, Cheney R, Wallace P, Loree T, Rigual N, Hicks W, Tan D
Head & neck 2004 Jun;26(6):544-9

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Natural killer and natural killer-T cells in psoriasis.

Cameron AL, Kirby B, Fei W, Griffiths CE
Archives of dermatological research 2002 Nov;294(8):363-9

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Blisters in the small intestinal mucosa of coeliac patients contain T cells positive for cyclooxygenase 2.

Kainulainen H, Rantala I, Collin P, Ruuska T, Päivärinne H, Halttunen T, Lindfors K, Kaukinen K, Mäki M
Gut 2002 Jan;50(1):84-9

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Involvement of the Fas/FasL pathway in the pathogenesis of germ cell tumours of the adult testis.

Kersemaekers AM, van Weeren PC, Oosterhuis JW, Looijenga LH
The Journal of pathology 2002 Apr;196(4):423-9

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human tonsil stained with CD57 antibody using peroxidase-conjugate and DAB chromogen. Note intense cell membrane staining of natural killer cells in the germinal center.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD57 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-12008) at a dilution of 2 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated secondary antibody for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of CD57 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD57 monoclonal antibody (Product # MA5-12008) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

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