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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB3236 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB3236, RRID:AB_10628167
- Product name
- Cd69 monoclonal antibody, clone H1.2F3
- Antibody type
- Monoclonal
- Description
- Hamster monoclonal antibody raised against Cd69.
- Isotype
- IgG
- Antibody clone number
- H1.2F3
- Storage
- Store at 4°C.
Submitted references The activation antigen CD69.
Regulation of RAG-1 and CD69 expression in the thymus during positive and negative selection.
The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex.
Ziegler SF, Ramsdell F, Alderson MR
Stem cells (Dayton, Ohio) 1994 Sep;12(5):456-65
Stem cells (Dayton, Ohio) 1994 Sep;12(5):456-65
Regulation of RAG-1 and CD69 expression in the thymus during positive and negative selection.
Brändle D, Müller S, Müller C, Hengartner H, Pircher H
European journal of immunology 1994 Jan;24(1):145-51
European journal of immunology 1994 Jan;24(1):145-51
The mouse CD69 gene. Structure, expression, and mapping to the NK gene complex.
Ziegler SF, Levin SD, Johnson L, Copeland NG, Gilbert DJ, Jenkins NA, Baker E, Sutherland GR, Feldhaus AL, Ramsdell F
Journal of immunology (Baltimore, Md. : 1950) 1994 Feb 1;152(3):1228-36
Journal of immunology (Baltimore, Md. : 1950) 1994 Feb 1;152(3):1228-36
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Fresh BALB/c spleen cells were activated by incubation for 20 hours with PMA + ionomycin, then stained with either hamster IgG-FITC (as an isotype control) or Cd69 monoclonal antibody, clone H1.2F3 (Cat # MAB3236)-FITC. Large, activated cells were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).
- Validation comment
- Flow Cytometry
