Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 50-0729-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD72 Monoclonal Antibody (J4-117), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody J4-117 reacts with human CD72, a 45 kDa type II transmembrane glycoprotein that belongs to the C-type lectin superfamily. This glycoprotein is expressed on B cells at all stages of maturation except for plasma cells. CD72 is also expressed on dendritic cells, mast cells, and some macrophages. This receptor contains an immune-receptor tyrosine-base inhibition motif (ITIM) that associates with a tyrosine phosphatase, SHP-1. CD72 negatively regulates the major signal transduction pathways activated by the B cell receptor (BCR). Studies have demonstrated that the ligand for CD72 is CD100 (also known as Semaphorin 4D). CD72 can also react with CD5 to provide signaling between T and B cells. Finally, this receptor also prevents naive B cells from producing Ig and differentiating into B cells, and plays a role in controlling autoimmunity by enhancing apoptosis after Ag-specific BCR stimulation.
- Antibody clone number
- J4-117
- Concentration
- 5 µL/Test
Submitted references Human CD72 splicing isoform responsible for resistance to systemic lupus erythematosus regulates serum immunoglobulin level and is localized in endoplasmic reticulum.
CD72 negatively regulates KIT-mediated responses in human mast cells.
CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72.
CD72 down-modulates BCR-induced signal transduction and diminishes survival in primary mature B lymphocytes.
Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment.
CD72-mediated suppression of human naive B cell differentiation by down-regulating X-box binding protein 1.
A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5.
Hitomi Y, Adachi T, Tsuchiya N, Honda Z, Tokunaga K, Tsubata T
BMC immunology 2012 Dec 26;13:72
BMC immunology 2012 Dec 26;13:72
CD72 negatively regulates KIT-mediated responses in human mast cells.
Kataoka TR, Kumanogoh A, Bandara G, Metcalfe DD, Gilfillan AM
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2468-75
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2468-75
CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72.
Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O
PloS one 2007 Sep 5;2(9):e818
PloS one 2007 Sep 5;2(9):e818
CD72 down-modulates BCR-induced signal transduction and diminishes survival in primary mature B lymphocytes.
Li DH, Tung JW, Tarner IH, Snow AL, Yukinari T, Ngernmaneepothong R, Martinez OM, Parnes JR
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5321-8
Journal of immunology (Baltimore, Md. : 1950) 2006 May 1;176(9):5321-8
Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment.
Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H
International immunology 2005 Oct;17(10):1277-82
International immunology 2005 Oct;17(10):1277-82
CD72-mediated suppression of human naive B cell differentiation by down-regulating X-box binding protein 1.
Yamazaki T, Nagumo H, Hayashi T, Sugane K, Agematsu K
European journal of immunology 2005 Aug;35(8):2325-34
European journal of immunology 2005 Aug;35(8):2325-34
A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5.
Bikah G, Lynd FM, Aruffo AA, Ledbetter JA, Bondada S
International immunology 1998 Aug;10(8):1185-96
International immunology 1998 Aug;10(8):1185-96
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD19 FITC (Product # 11-0199-42) and Mouse IgG2b K Isotype Control eFluor® 660 (Product # 50-4732-82) (left) or Anti-Human CD72 eFluor® 660 (right). Cells in the lymphocyte gate were used for analysis.