MAB5405-100

antibody from R&D Systems

Targeting: CD72 CD72b, LYB2

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB5405-100

Provider

R&D Systems

Product name

Human CD72 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human CD72 in direct ELISAs.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P21854


Isotype

IgG

Antibody clone number

982405

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CD72 in Ramos Human Cell Line. CD72 was detected in immersion fixed Ramos human Burkitt's lymphoma cell line using Mouse Anti-Human CD72 Monoclonal Antibody (Catalog # MAB5405) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CD72 in Human Ramos Cell Line by Flow Cytometry. Human Ramos Burkitt's lymphoma cell line was stained with Mouse Anti-Human CD72 Monoclonal Antibody (Catalog # MAB5405, filled histogram) or Mouse IgG2A Isotype Control (Catalog # MAB003, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.