Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [5]
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Validation data
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- Product number
- NBP2-21792 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD82/Kai-1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody C33 reacts with CD82, a widely expressed cell surface protein of the tetraspanin family. CD82 is also found in endosome/lysosome compartments.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of human tonsil (paraffin-embedded sections) with anti-CD82 (C33).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) APC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Flow Cytometry: [PerCP] [NBP2-21792PCP] - A cell surface (A) and intracellular stain (B) was performed on Daudi cells with CD82/Kai-1 (C33) antibody NBP2-21792PCP along with a matched isotype control NB600-986PCP. The cell surface stain was performed in an antibody dilution of 5 ug/mL and incubated at RT for 20 minutes. Prior to intracellular staining, the cells were fixed with 4% PFA and then permeablized with 0.1 % saponin. The cells were then incubated in an antibody dilution of 5 ug/mL at RT for 30 minutes. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex. Image using the PerCP form of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) PE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Staining of CD82 on human peripheral blood cells with anti-CD82 (C33) FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD82/Kai-1 Antibody (C33) [NBP2-21792] - Analysis (surface staining) of CD82 on human peripheral blood cells with anti-CD82 (C33) biotin, streptavidin/PE.