Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA1-9077 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PAI1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with canine based on sequence homology. This antibody is tested in Peptide ELISA: antibody detection limit dilution 64,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PAI1 by a PAI1 monoclonal antibody (Product # PA1-9077) at a concentration of 0.1 µg/mL. A549 (A), HepG2 (B) and HeLa (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of SERPINE1 in human liver lysate using Product # PA1-9077.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA of PAI1 using two PAI1 antibodies. The reporter antibody (Product # PA1-9077) was used at a concentration of 1.5 µg/ml and the reporter antibody was used at a concentration of 2.5 µg/ml.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PAI1 in Human Placenta using a PAI1 monoclonal antibody (Product #PA1-9077) at 4 µg/mL. The Human Placenta tissue section was paraffin embeded and detected using steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.