Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 20805-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HTATSF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSGTNLDGN DEFDEQLRMQ ELYGDGKDGD TQTDAGGEPD SLGQQPTDTP YEWDLDKKAW FPKITEDFIA TYQANYGFSN DGASSSTANV EDVHARTAEE PPQEKAPEPT DARKKGEKRK AESGWFHVEE DRNTNVYVSG LPPDITVDEF IQLMSKFGII MRDPQTEEFK VKLYKDNQGN LKGDGLCCYL KRESVELALK LLDEDEIRGY KLHVEVAKFQ LKGEYDASKK KKKCKDYKKK LSMQQKQLDW RPERRAGPSR MRHERVVIIK NMFHPMDFED DPLVLNEIRE DLRVECSKFG QIRKLLLFDR HPDGVASVSF RDPEEADYCI QTLDGRWFGG RQITAQAWDG T (1-350 aa encoded by BC009896)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 20805-1-AP ( HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 20805-1-AP (HTATSF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using HTATSF1 antibody 20805-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using HTATSF1 antibody 20805-1-AP at 1:50 dilution and FITC- labeled FITC-Donkey anti-Rabbit IgG (green).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 20805-1-AP (HTATSF1 antibody) at dilution of 1:100 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HTATSF1 (IP:20805-1-AP, 4ug; Detection:20805-1-AP 1:2000) with HepG2 cells lysate 2400ug.