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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF7284 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Ets-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Ets-1 in Western blots and detects recombinant human Ets-1 in direct ELISAs.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P14921- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Ets-1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, human thymus tissue, mouse thymus tissue, and rat CD4+ T cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat Ets-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7284) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Ets-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Ets-1 in Human Ovary. Ets-1 was detected in immersion fixed paraffin-embedded sections of human ovary using Sheep Anti-Human/Mouse/Rat Ets-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7284) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
