Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF364 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL17/TARC Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human CCL17/TARC in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse TARC is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q92583
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
miR-24-3p Is Overexpressed in Hodgkin Lymphoma and Protects Hodgkin and Reed-Sternberg Cells from Apoptosis.
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Pathogenesis of cBFL in common with IPF? Correlation of IP-10/TARC ratio with histological patterns.
Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
Gene expression analysis of dendritic/Langerhans cells and Langerhans cell histiocytosis.
Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
TARC augments TNF-alpha-induced CTACK production in keratinocytes.
Common and differential chemokine expression patterns in rs cells of NLP, EBV positive and negative classical Hodgkin lymphomas.
Rump L, Mattey DL, Kehoe O, Middleton J
Cytokine 2017 Sep;97:133-140
Cytokine 2017 Sep;97:133-140
miR-24-3p Is Overexpressed in Hodgkin Lymphoma and Protects Hodgkin and Reed-Sternberg Cells from Apoptosis.
Yuan Y, Kluiver J, Koerts J, de Jong D, Rutgers B, Abdul Razak FR, Terpstra M, Plaat BE, Nolte IM, Diepstra A, Visser L, Kok K, van den Berg A
The American journal of pathology 2017 Jun;187(6):1343-1355
The American journal of pathology 2017 Jun;187(6):1343-1355
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2886-98
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2886-98
Pathogenesis of cBFL in common with IPF? Correlation of IP-10/TARC ratio with histological patterns.
Kishi M, Miyazaki Y, Jinta T, Furusawa H, Ohtani Y, Inase N, Yoshizawa Y
Thorax 2008 Sep;63(9):810-6
Thorax 2008 Sep;63(9):810-6
Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, van den Berg A
Blood 2008 Feb 15;111(4):2339-46
Blood 2008 Feb 15;111(4):2339-46
Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
Heijink IH, Kies PM, Kauffman HF, Postma DS, van Oosterhout AJ, Vellenga E
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):7678-85
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):7678-85
Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
Heijink IH, Kies PM, Kauffman HF, Postma DS, van Oosterhout AJ, Vellenga E
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):7678-85
Journal of immunology (Baltimore, Md. : 1950) 2007 Jun 15;178(12):7678-85
Gene expression analysis of dendritic/Langerhans cells and Langerhans cell histiocytosis.
Rust R, Kluiver J, Visser L, Harms G, Blokzijl T, Kamps W, Poppema S, van den Berg A
The Journal of pathology 2006 Aug;209(4):474-83
The Journal of pathology 2006 Aug;209(4):474-83
Classification of distinct subtypes of peripheral T-cell lymphoma unspecified, identified by chemokine and chemokine receptor expression: Analysis of prognosis.
Ohshima K, Karube K, Kawano R, Tsuchiya T, Suefuji H, Yamaguchi T, Suzumiya J, Kikuchii M
International journal of oncology 2004 Sep;25(3):605-13
International journal of oncology 2004 Sep;25(3):605-13
TARC augments TNF-alpha-induced CTACK production in keratinocytes.
Vestergaard C, Johansen C, Christensen U, Just H, Hohwy T, Deleuran M
Experimental dermatology 2004 Sep;13(9):551-7
Experimental dermatology 2004 Sep;13(9):551-7
Common and differential chemokine expression patterns in rs cells of NLP, EBV positive and negative classical Hodgkin lymphomas.
Maggio EM, Van Den Berg A, Visser L, Diepstra A, Kluiver J, Emmens R, Poppema S
International journal of cancer 2002 Jun 10;99(5):665-72
International journal of cancer 2002 Jun 10;99(5):665-72
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL17/TARC in Human Spleen. CCL17/TARC was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL17/TARC and Neutralization by Human CCL17/TARC Antibody. Recombinant Human CCL17/TARC (Catalog # 364-DN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL17/ TARC (0.01 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human CCL17/TARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF364). The ND50 is typically 0.3-1.5 µg/mL.