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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB6840 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TORC3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse TORC3 in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant human TORC3 and no cross-reactivity with recombinant mouse (rm) TORC1 or rmTORC2 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q91X84- Isotype
- IgG
- Antibody clone number
- 643729
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse TORC3 by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse TORC3 Monoclonal Antibody (Catalog # MAB6840) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TORC3 at approximately 83 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
