NBP1-46832

antibody from Novus Biologicals

Targeting: CRTC3 FLJ21868

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-46832

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-46832, RRID:AB_10009895

Product name

Rabbit Polyclonal TORC3/CRTC3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: TORC3/CRTC3 Antibody [NBP1-46832] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TORC3 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TORC3 was also immunoprecipitated by rabbit anti-TORC3 antibody BL9829, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TORC3/CRTC3 Antibody [NBP1-46832] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TORC3 used at a dilution of 1:200 (1ug/ml). Detection: DAB