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LearnGTX112898
antibody from GeneTex
Targeting: CDKN1A
CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX112898 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112898, RRID:AB_10617567
- Product name
- p21 Cip1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Chicken/Avian
- Host
- Rabbit
Submitted references MiR-16-5p targets SESN1 to regulate the p53 signaling pathway, affecting myoblast proliferation and apoptosis, and is involved in myoblast differentiation.
Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1.
Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1.
CARP, a myostatin-downregulated gene in CFM Cells, is a novel essential positive regulator of myogenesis.
Cai B, Ma M, Chen B, Li Z, Abdalla BA, Nie Q, Zhang X
Cell death & disease 2018 Mar 6;9(3):367
Cell death & disease 2018 Mar 6;9(3):367
Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1.
Lee WY, Lee WT, Cheng CH, Chen KC, Chou CM, Chung CH, Sun MS, Cheng HW, Ho MN, Lin CW
Oncotarget 2015 Sep 29;6(29):27580-95
Oncotarget 2015 Sep 29;6(29):27580-95
Repositioning antipsychotic chlorpromazine for treating colorectal cancer by inhibiting sirtuin 1.
Lee WY, Lee WT, Cheng CH, Chen KC, Chou CM, Chung CH, Sun MS, Cheng HW, Ho MN, Lin CW
Oncotarget 2015 Sep 29;6(29):27580-95
Oncotarget 2015 Sep 29;6(29):27580-95
CARP, a myostatin-downregulated gene in CFM Cells, is a novel essential positive regulator of myogenesis.
Ma G, Wang H, Gu X, Li W, Zhang X, Cui L, Li Y, Zhang Y, Zhao B, Li K
International journal of biological sciences 2014;10(3):309-20
International journal of biological sciences 2014;10(3):309-20
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HCT116 cells with 30 ?M cisplatin treatment for 0 hr B: HCT116 cells with 30 ?M cisplatin treatment for 24 hr 12% SDS PAGE GTX112898 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody (GTX112898) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and p21 Cip1 knockout (KO) HeLa cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with p21 Cip1 antibody (GTX112898) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- p21 Cip1 antibody detects p21 Cip1 protein at nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 30 £gM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX112898) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- p21 Cip1 antibody detects CDKN1A protein at nucleus on SkHep1xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1xenograft. p21 Cip1 antibody (GTX112898) dilution: 1:500.
