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LearnF54937
antibody from NSJ Bioreagents
Targeting: CDKN1A
CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F54937 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- p21 Antibody / CDKN1A
- Antibody type
- Polyclonal
- Description
- Purified
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.08 µl, 0.4 µl
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the p21WAF1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human T-47D cell lysate with p21WAF1 antibody. Expected molecular weight: 18-21 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HEK293 cells with p21WAF1 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human cancer tissue with p21WAF1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human HEK293 cells with p21WAF1 antibody; Blue=isotype control, Green= p21WAF1 antibody.
