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LearnMA5-14350
antibody from Invitrogen Antibodies
Targeting: CDKN1A
CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [70]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [2]
- Other assay [3]
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- Product number
- MA5-14350 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-p21 Monoclonal Antibody (CP74), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- MA5-14350 targets p21WAF11 in FACS, IHC (P), IP, ICC/IF and WB applications and shows reactivity with Human, Mouse and Rat samples. The MA5-14350 immunogen is full length human recombinant p21 protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- CP74
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Inauhzin sensitizes p53-dependent cytotoxicity and tumor suppression of chemotherapeutic agents.
Positive regulation of p53 stability and activity by the deubiquitinating enzyme Otubain 1.
The p53-reactivating small-molecule RITA enhances cisplatin-induced cytotoxicity and apoptosis in head and neck cancer.
MicroRNA-223 regulates FOXO1 expression and cell proliferation.
CyclinD1, p53, E-cadherin, and VEGF discordant expression in paired regional metastatic lymph nodes of esophageal squamous cell carcinoma: a tissue array analysis.
Interplay between ribosomal protein S27a and MDM2 protein in p53 activation in response to ribosomal stress.
p53-Reactivating small molecules induce apoptosis and enhance chemotherapeutic cytotoxicity in head and neck squamous cell carcinoma.
Aurora A selective inhibitor MLN8237 suppresses the growth and survival of HTLV-1-infected T-cells in vitro.
The ATDC (TRIM29) protein binds p53 and antagonizes p53-mediated functions.
p21CDKN1A participates in base excision repair by regulating the activity of poly(ADP-ribose) polymerase-1.
4'-Chloro-3,5-dihydroxystilbene, a resveratrol derivative, induces lung cancer cell death.
Human testicular orphan receptor 4 enhances thyroid hormone receptor signaling.
EBV-positive Hodgkin lymphoma is associated with suppression of p21cip1/waf1 and a worse prognosis.
Histone deacetylase 9 (HDAC9) regulates the functions of the ATDC (TRIM29) protein.
HER2 carboxyl-terminal fragments regulate cell migration and cortactin phosphorylation.
ERCC1 expression and RAD51B activity correlate with cell cycle response to platinum drug treatment not DNA repair.
Multiple division cycles and long-term survival of hepatocytes are distinctly regulated by extracellular signal-regulated kinases ERK1 and ERK2.
Development of a three-dimensional culture model of prostatic epithelial cells and its use for the study of epithelial-mesenchymal transition and inhibition of PI3K pathway in prostate cancer.
Degradation of p21CDKN1A after DNA damage is independent of type of lesion, and is not required for DNA repair.
Puerariae radix isoflavones and their metabolites inhibit growth and induce apoptosis in breast cancer cells.
Loss of p21 CDKN1A impairs entry to quiescence and activates a DNA damage response in normal fibroblasts induced to quiescence.
p53-dependent antiproliferative and antitumor effect of novel alkyl series of diorganotin(IV) compounds.
Oligomeric proanthocyanidin complexes (OPC) exert anti-proliferative and pro-apoptotic effects on prostate cancer cells.
Orphan nuclear receptor estrogen-related receptor-beta suppresses in vitro and in vivo growth of prostate cancer cells via p21(WAF1/CIP1) induction and as a potential therapeutic target in prostate cancer.
Apigenin-induced prostate cancer cell death is initiated by reactive oxygen species and p53 activation.
A 39 kDa fragment of endogenous ASK1 suggests specific cleavage not degradation by the proteasome.
Interaction of p21(CDKN1A) with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair.
hTERT expression in colorectal adenocarcinoma: correlations with p21, p53 expressions and clinicopathological features.
Aberrant expression of nucleostemin activates p53 and induces cell cycle arrest via inhibition of MDM2.
Defining targets of modulation of human tumor cell response to cisplatin.
Involvement of the role of Chk1 in lithium-induced G2/M phase cell cycle arrest in hepatocellular carcinoma cells.
Defective cell cycle checkpoint functions in melanoma are associated with altered patterns of gene expression.
MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2.
Magnesium deficiency accelerates cellular senescence in cultured human fibroblasts.
AZD6244 and doxorubicin induce growth suppression and apoptosis in mouse models of hepatocellular carcinoma.
Influence of glutathione on the induction of chromosome aberrations, delay in cell cycle kinetics and cell cycle regulator proteins in irradiated mouse bone marrow cells.
Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells.
Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells.
Role of microphthalmia transcription factor (Mitf) in melanoma differentiation.
Antitumor activity of CTFB, a novel anticancer agent, is associated with the down-regulation of nuclear factor-kappaB expression and proteasome activation in head and neck squamous carcinoma cell lines.
Induction of cullin 7 by DNA damage attenuates p53 function.
Overexpressed TP73 induces apoptosis in medulloblastoma.
D-501036, a novel selenophene-based triheterocycle derivative, exhibits potent in vitro and in vivo antitumoral activity which involves DNA damage and ataxia telangiectasia-mutated nuclear protein kinase activation.
Histone deacetylase inhibitors induce the degradation of the t(8;21) fusion oncoprotein.
p53-Dependent but ATM-independent inhibition of DNA synthesis and G2 arrest in cadmium-treated human fibroblasts.
Inducible microRNA expression by an all-in-one episomal vector system.
Replication-dependent DNA damage response triggered by roscovitine induces an uncoupling of DNA replication proteins.
UV Induces p21 rapid turnover independently of ubiquitin and Skp2.
Coupling caspase cleavage and ubiquitin-proteasome-dependent degradation of SSRP1 during apoptosis.
Prolonged exposure to arecoline arrested human KB epithelial cell growth: regulatory mechanisms of cell cycle and apoptosis.
N-all-trans-retinoyl-L-proline inhibits metastatic potential of hepatocellular carcinoma cells.
Inhibitory effects of N-(4-hydrophenyl) retinamide on liver cancer and malignant melanoma cells.
The chemoprotective agent N-acetylcysteine blocks cisplatin-induced apoptosis through caspase signaling pathway.
Xrcc3 induces cisplatin resistance by stimulation of Rad51-related recombinational repair, S-phase checkpoint activation, and reduced apoptosis.
A mechanistic basis for the role of cycle arrest in the genetic toxicology of the dietary carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP).
The mitogen-activated protein kinase kinase kinase dual leucine zipper-bearing kinase (DLK) acts as a key regulator of keratinocyte terminal differentiation.
Cell-type-specific responses to chemotherapeutics in breast cancer.
Role for BRG1 in cell cycle control and tumor suppression.
Functional and physical interaction of the human ARF tumor suppressor with Tat-binding protein-1.
P27Kip1 and p21Cip1 are not required for the formation of active D cyclin-cdk4 complexes.
Caffeine and human DNA metabolism: the magic and the mystery.
S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes.
Mammary epithelial cells of PR-A transgenic mice exhibit distinct alterations in gene expression and growth potential associated with transformation.
Delineation of the molecular basis for selenium-induced growth arrest in human prostate cancer cells by oligonucleotide array.
MDM2 promotes p21waf1/cip1 proteasomal turnover independently of ubiquitylation.
G(1) and G(2) cell-cycle arrest following microtubule depolymerization in human breast cancer cells.
E2F4/5 and p107 as Smad cofactors linking the TGFbeta receptor to c-myc repression.
Differential roles of p21(Waf1) and p27(Kip1) in modulating chemosensitivity and their possible application in drug discovery studies.
Notch signaling induces cell cycle arrest in small cell lung cancer cells.
Comparison of potential markers of farnesyltransferase inhibition.
Zhang Y, Zhang Q, Zeng SX, Hao Q, Lu H
Neoplasia (New York, N.Y.) 2013 May;15(5):523-34
Neoplasia (New York, N.Y.) 2013 May;15(5):523-34
Positive regulation of p53 stability and activity by the deubiquitinating enzyme Otubain 1.
Sun XX, Challagundla KB, Dai MS
The EMBO journal 2012 Feb 1;31(3):576-92
The EMBO journal 2012 Feb 1;31(3):576-92
The p53-reactivating small-molecule RITA enhances cisplatin-induced cytotoxicity and apoptosis in head and neck cancer.
Roh JL, Ko JH, Moon SJ, Ryu CH, Choi JY, Koch WM
Cancer letters 2012 Dec 1;325(1):35-41
Cancer letters 2012 Dec 1;325(1):35-41
MicroRNA-223 regulates FOXO1 expression and cell proliferation.
Wu L, Li H, Jia CY, Cheng W, Yu M, Peng M, Zhu Y, Zhao Q, Dong YW, Shao K, Wu A, Wu XZ
FEBS letters 2012 Apr 5;586(7):1038-43
FEBS letters 2012 Apr 5;586(7):1038-43
CyclinD1, p53, E-cadherin, and VEGF discordant expression in paired regional metastatic lymph nodes of esophageal squamous cell carcinoma: a tissue array analysis.
Luo KJ, Hu Y, Wen J, Fu JH
Journal of surgical oncology 2011 Sep 1;104(3):236-43
Journal of surgical oncology 2011 Sep 1;104(3):236-43
Interplay between ribosomal protein S27a and MDM2 protein in p53 activation in response to ribosomal stress.
Sun XX, DeVine T, Challagundla KB, Dai MS
The Journal of biological chemistry 2011 Jul 1;286(26):22730-41
The Journal of biological chemistry 2011 Jul 1;286(26):22730-41
p53-Reactivating small molecules induce apoptosis and enhance chemotherapeutic cytotoxicity in head and neck squamous cell carcinoma.
Roh JL, Kang SK, Minn I, Califano JA, Sidransky D, Koch WM
Oral oncology 2011 Jan;47(1):8-15
Oral oncology 2011 Jan;47(1):8-15
Aurora A selective inhibitor MLN8237 suppresses the growth and survival of HTLV-1-infected T-cells in vitro.
Tomita M, Mori N
Cancer science 2010 May;101(5):1204-11
Cancer science 2010 May;101(5):1204-11
The ATDC (TRIM29) protein binds p53 and antagonizes p53-mediated functions.
Yuan Z, Villagra A, Peng L, Coppola D, Glozak M, Sotomayor EM, Chen J, Lane WS, Seto E
Molecular and cellular biology 2010 Jun;30(12):3004-15
Molecular and cellular biology 2010 Jun;30(12):3004-15
p21CDKN1A participates in base excision repair by regulating the activity of poly(ADP-ribose) polymerase-1.
Cazzalini O, Donà F, Savio M, Tillhon M, Maccario C, Perucca P, Stivala LA, Scovassi AI, Prosperi E
DNA repair 2010 Jun 4;9(6):627-35
DNA repair 2010 Jun 4;9(6):627-35
4'-Chloro-3,5-dihydroxystilbene, a resveratrol derivative, induces lung cancer cell death.
Wu JY, Tsai KW, Shee JJ, Li YZ, Chen CH, Chuang JJ, Liu YW
Acta pharmacologica Sinica 2010 Jan;31(1):81-92
Acta pharmacologica Sinica 2010 Jan;31(1):81-92
Human testicular orphan receptor 4 enhances thyroid hormone receptor signaling.
Huang YH, Liao CH, Chen RN, Liao CJ, Lin KH
Journal of cellular physiology 2010 Feb;222(2):347-56
Journal of cellular physiology 2010 Feb;222(2):347-56
EBV-positive Hodgkin lymphoma is associated with suppression of p21cip1/waf1 and a worse prognosis.
Liu TY, Wu SJ, Huang MH, Lo FY, Tsai MH, Tsai CH, Hsu SM, Lin CW
Molecular cancer 2010 Feb 9;9:32
Molecular cancer 2010 Feb 9;9:32
Histone deacetylase 9 (HDAC9) regulates the functions of the ATDC (TRIM29) protein.
Yuan Z, Peng L, Radhakrishnan R, Seto E
The Journal of biological chemistry 2010 Dec 10;285(50):39329-38
The Journal of biological chemistry 2010 Dec 10;285(50):39329-38
HER2 carboxyl-terminal fragments regulate cell migration and cortactin phosphorylation.
García-Castillo J, Pedersen K, Angelini PD, Bech-Serra JJ, Colomé N, Cunningham MP, Parra-Palau JL, Canals F, Baselga J, Arribas J
The Journal of biological chemistry 2009 Sep 11;284(37):25302-13
The Journal of biological chemistry 2009 Sep 11;284(37):25302-13
ERCC1 expression and RAD51B activity correlate with cell cycle response to platinum drug treatment not DNA repair.
Stordal B, Davey R
Cancer chemotherapy and pharmacology 2009 Mar;63(4):661-72
Cancer chemotherapy and pharmacology 2009 Mar;63(4):661-72
Multiple division cycles and long-term survival of hepatocytes are distinctly regulated by extracellular signal-regulated kinases ERK1 and ERK2.
Frémin C, Bessard A, Ezan F, Gailhouste L, Régeard M, Le Seyec J, Gilot D, Pagès G, Pouysségur J, Langouët S, Baffet G
Hepatology (Baltimore, Md.) 2009 Mar;49(3):930-9
Hepatology (Baltimore, Md.) 2009 Mar;49(3):930-9
Development of a three-dimensional culture model of prostatic epithelial cells and its use for the study of epithelial-mesenchymal transition and inhibition of PI3K pathway in prostate cancer.
Chu JH, Yu S, Hayward SW, Chan FL
The Prostate 2009 Mar 1;69(4):428-42
The Prostate 2009 Mar 1;69(4):428-42
Degradation of p21CDKN1A after DNA damage is independent of type of lesion, and is not required for DNA repair.
Savio M, Coppa T, Cazzalini O, Perucca P, Necchi D, Nardo T, Stivala LA, Prosperi E
DNA repair 2009 Jul 4;8(7):778-85
DNA repair 2009 Jul 4;8(7):778-85
Puerariae radix isoflavones and their metabolites inhibit growth and induce apoptosis in breast cancer cells.
Lin YJ, Hou YC, Lin CH, Hsu YA, Sheu JJ, Lai CH, Chen BH, Lee Chao PD, Wan L, Tsai FJ
Biochemical and biophysical research communications 2009 Jan 23;378(4):683-8
Biochemical and biophysical research communications 2009 Jan 23;378(4):683-8
Loss of p21 CDKN1A impairs entry to quiescence and activates a DNA damage response in normal fibroblasts induced to quiescence.
Perucca P, Cazzalini O, Madine M, Savio M, Laskey RA, Vannini V, Prosperi E, Stivala LA
Cell cycle (Georgetown, Tex.) 2009 Jan 1;8(1):105-14
Cell cycle (Georgetown, Tex.) 2009 Jan 1;8(1):105-14
p53-dependent antiproliferative and antitumor effect of novel alkyl series of diorganotin(IV) compounds.
Koch B, Basu Baul TS, Chatterjee A
Investigational new drugs 2009 Aug;27(4):319-26
Investigational new drugs 2009 Aug;27(4):319-26
Oligomeric proanthocyanidin complexes (OPC) exert anti-proliferative and pro-apoptotic effects on prostate cancer cells.
Neuwirt H, Arias MC, Puhr M, Hobisch A, Culig Z
The Prostate 2008 Nov 1;68(15):1647-54
The Prostate 2008 Nov 1;68(15):1647-54
Orphan nuclear receptor estrogen-related receptor-beta suppresses in vitro and in vivo growth of prostate cancer cells via p21(WAF1/CIP1) induction and as a potential therapeutic target in prostate cancer.
Yu S, Wong YC, Wang XH, Ling MT, Ng CF, Chen S, Chan FL
Oncogene 2008 May 22;27(23):3313-28
Oncogene 2008 May 22;27(23):3313-28
Apigenin-induced prostate cancer cell death is initiated by reactive oxygen species and p53 activation.
Shukla S, Gupta S
Free radical biology & medicine 2008 May 15;44(10):1833-45
Free radical biology & medicine 2008 May 15;44(10):1833-45
A 39 kDa fragment of endogenous ASK1 suggests specific cleavage not degradation by the proteasome.
Stordal B, Davey R
IUBMB life 2008 Mar;60(3):180-4
IUBMB life 2008 Mar;60(3):180-4
Interaction of p21(CDKN1A) with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair.
Cazzalini O, Perucca P, Savio M, Necchi D, Bianchi L, Stivala LA, Ducommun B, Scovassi AI, Prosperi E
Nucleic acids research 2008 Mar;36(5):1713-22
Nucleic acids research 2008 Mar;36(5):1713-22
hTERT expression in colorectal adenocarcinoma: correlations with p21, p53 expressions and clinicopathological features.
Lam AK, Ong K, Ho YH
International journal of colorectal disease 2008 Jun;23(6):587-94
International journal of colorectal disease 2008 Jun;23(6):587-94
Aberrant expression of nucleostemin activates p53 and induces cell cycle arrest via inhibition of MDM2.
Dai MS, Sun XX, Lu H
Molecular and cellular biology 2008 Jul;28(13):4365-76
Molecular and cellular biology 2008 Jul;28(13):4365-76
Defining targets of modulation of human tumor cell response to cisplatin.
Beretta GL, Gatti L, Corna E, Carenini N, Zunino F, Perego P
Journal of inorganic biochemistry 2008 Jul;102(7):1406-15
Journal of inorganic biochemistry 2008 Jul;102(7):1406-15
Involvement of the role of Chk1 in lithium-induced G2/M phase cell cycle arrest in hepatocellular carcinoma cells.
Wang XM, Li J, Feng XC, Wang Q, Guan DY, Shen ZH
Journal of cellular biochemistry 2008 Jul 1;104(4):1181-91
Journal of cellular biochemistry 2008 Jul 1;104(4):1181-91
Defective cell cycle checkpoint functions in melanoma are associated with altered patterns of gene expression.
Kaufmann WK, Nevis KR, Qu P, Ibrahim JG, Zhou T, Zhou Y, Simpson DA, Helms-Deaton J, Cordeiro-Stone M, Moore DT, Thomas NE, Hao H, Liu Z, Shields JM, Scott GA, Sharpless NE
The Journal of investigative dermatology 2008 Jan;128(1):175-87
The Journal of investigative dermatology 2008 Jan;128(1):175-87
MDMX promotes proteasomal turnover of p21 at G1 and early S phases independently of, but in cooperation with, MDM2.
Jin Y, Zeng SX, Sun XX, Lee H, Blattner C, Xiao Z, Lu H
Molecular and cellular biology 2008 Feb;28(4):1218-29
Molecular and cellular biology 2008 Feb;28(4):1218-29
Magnesium deficiency accelerates cellular senescence in cultured human fibroblasts.
Killilea DW, Ames BN
Proceedings of the National Academy of Sciences of the United States of America 2008 Apr 15;105(15):5768-73
Proceedings of the National Academy of Sciences of the United States of America 2008 Apr 15;105(15):5768-73
AZD6244 and doxorubicin induce growth suppression and apoptosis in mouse models of hepatocellular carcinoma.
Huynh H, Chow PK, Soo KC
Molecular cancer therapeutics 2007 Sep;6(9):2468-76
Molecular cancer therapeutics 2007 Sep;6(9):2468-76
Influence of glutathione on the induction of chromosome aberrations, delay in cell cycle kinetics and cell cycle regulator proteins in irradiated mouse bone marrow cells.
Ray S, Chatterjee A
International journal of radiation biology 2007 May;83(5):347-54
International journal of radiation biology 2007 May;83(5):347-54
Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21(Cip1) and p27(Kip1) in human pancreatic adenocarcinoma cells.
Wiseman DA, Werner SR, Crowell PL
The Journal of pharmacology and experimental therapeutics 2007 Mar;320(3):1163-70
The Journal of pharmacology and experimental therapeutics 2007 Mar;320(3):1163-70
Requirements for Cdk7 in the assembly of Cdk1/cyclin B and activation of Cdk2 revealed by chemical genetics in human cells.
Larochelle S, Merrick KA, Terret ME, Wohlbold L, Barboza NM, Zhang C, Shokat KM, Jallepalli PV, Fisher RP
Molecular cell 2007 Mar 23;25(6):839-50
Molecular cell 2007 Mar 23;25(6):839-50
Role of microphthalmia transcription factor (Mitf) in melanoma differentiation.
Lekmine F, Chang CK, Sethakorn N, Das Gupta TK, Salti GI
Biochemical and biophysical research communications 2007 Mar 16;354(3):830-5
Biochemical and biophysical research communications 2007 Mar 16;354(3):830-5
Antitumor activity of CTFB, a novel anticancer agent, is associated with the down-regulation of nuclear factor-kappaB expression and proteasome activation in head and neck squamous carcinoma cell lines.
Skvortsov S, Skvortsova I, Stasyk T, Schiefermeier N, Neher A, Gunkel AR, Bonn GK, Huber LA, Lukas P, Pleiman CM, Zwierzina H
Molecular cancer therapeutics 2007 Jun;6(6):1898-908
Molecular cancer therapeutics 2007 Jun;6(6):1898-908
Induction of cullin 7 by DNA damage attenuates p53 function.
Jung P, Verdoodt B, Bailey A, Yates JR 3rd, Menssen A, Hermeking H
Proceedings of the National Academy of Sciences of the United States of America 2007 Jul 3;104(27):11388-93
Proceedings of the National Academy of Sciences of the United States of America 2007 Jul 3;104(27):11388-93
Overexpressed TP73 induces apoptosis in medulloblastoma.
Castellino RC, De Bortoli M, Lin LL, Skapura DG, Rajan JA, Adesina AM, Perlaky L, Irwin MS, Kim JY
BMC cancer 2007 Jul 12;7:127
BMC cancer 2007 Jul 12;7:127
D-501036, a novel selenophene-based triheterocycle derivative, exhibits potent in vitro and in vivo antitumoral activity which involves DNA damage and ataxia telangiectasia-mutated nuclear protein kinase activation.
Juang SH, Lung CC, Hsu PC, Hsu KS, Li YC, Hong PC, Shiah HS, Kuo CC, Huang CW, Wang YC, Huang L, Chen TS, Chen SF, Fu KC, Hsu CL, Lin MJ, Chang CJ, Ashendel CL, Chan TC, Chou KM, Chang JY
Molecular cancer therapeutics 2007 Jan;6(1):193-202
Molecular cancer therapeutics 2007 Jan;6(1):193-202
Histone deacetylase inhibitors induce the degradation of the t(8;21) fusion oncoprotein.
Yang G, Thompson MA, Brandt SJ, Hiebert SW
Oncogene 2007 Jan 4;26(1):91-101
Oncogene 2007 Jan 4;26(1):91-101
p53-Dependent but ATM-independent inhibition of DNA synthesis and G2 arrest in cadmium-treated human fibroblasts.
Cao F, Zhou T, Simpson D, Zhou Y, Boyer J, Chen B, Jin T, Cordeiro-Stone M, Kaufmann W
Toxicology and applied pharmacology 2007 Jan 15;218(2):174-85
Toxicology and applied pharmacology 2007 Jan 15;218(2):174-85
Inducible microRNA expression by an all-in-one episomal vector system.
Epanchintsev A, Jung P, Menssen A, Hermeking H
Nucleic acids research 2006;34(18):e119
Nucleic acids research 2006;34(18):e119
Replication-dependent DNA damage response triggered by roscovitine induces an uncoupling of DNA replication proteins.
Savio M, Cerri M, Cazzalini O, Perucca P, Stivala LA, Pichierri P, Franchitto A, Meijer L, Prosperi E
Cell cycle (Georgetown, Tex.) 2006 Sep;5(18):2153-9
Cell cycle (Georgetown, Tex.) 2006 Sep;5(18):2153-9
UV Induces p21 rapid turnover independently of ubiquitin and Skp2.
Lee H, Zeng SX, Lu H
The Journal of biological chemistry 2006 Sep 15;281(37):26876-83
The Journal of biological chemistry 2006 Sep 15;281(37):26876-83
Coupling caspase cleavage and ubiquitin-proteasome-dependent degradation of SSRP1 during apoptosis.
Landais I, Lee H, Lu H
Cell death and differentiation 2006 Nov;13(11):1866-78
Cell death and differentiation 2006 Nov;13(11):1866-78
Prolonged exposure to arecoline arrested human KB epithelial cell growth: regulatory mechanisms of cell cycle and apoptosis.
Lee PH, Chang MC, Chang WH, Wang TM, Wang YJ, Hahn LJ, Ho YS, Lin CY, Jeng JH
Toxicology 2006 Mar 15;220(2-3):81-9
Toxicology 2006 Mar 15;220(2-3):81-9
N-all-trans-retinoyl-L-proline inhibits metastatic potential of hepatocellular carcinoma cells.
Wu XZ, Shi PC, Hu P, Chen Y, Ding SS
Cell biology international 2006 Aug;30(8):672-80
Cell biology international 2006 Aug;30(8):672-80
Inhibitory effects of N-(4-hydrophenyl) retinamide on liver cancer and malignant melanoma cells.
Wu XZ, Zhang L, Shi BZ, Hu P
World journal of gastroenterology 2005 Oct 7;11(37):5763-9
World journal of gastroenterology 2005 Oct 7;11(37):5763-9
The chemoprotective agent N-acetylcysteine blocks cisplatin-induced apoptosis through caspase signaling pathway.
Wu YJ, Muldoon LL, Neuwelt EA
The Journal of pharmacology and experimental therapeutics 2005 Feb;312(2):424-31
The Journal of pharmacology and experimental therapeutics 2005 Feb;312(2):424-31
Xrcc3 induces cisplatin resistance by stimulation of Rad51-related recombinational repair, S-phase checkpoint activation, and reduced apoptosis.
Xu ZY, Loignon M, Han FY, Panasci L, Aloyz R
The Journal of pharmacology and experimental therapeutics 2005 Aug;314(2):495-505
The Journal of pharmacology and experimental therapeutics 2005 Aug;314(2):495-505
A mechanistic basis for the role of cycle arrest in the genetic toxicology of the dietary carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP).
Creton S, Zhu H, Gooderham NJ
Toxicological sciences : an official journal of the Society of Toxicology 2005 Apr;84(2):335-43
Toxicological sciences : an official journal of the Society of Toxicology 2005 Apr;84(2):335-43
The mitogen-activated protein kinase kinase kinase dual leucine zipper-bearing kinase (DLK) acts as a key regulator of keratinocyte terminal differentiation.
Robitaille H, Proulx R, Robitaille K, Blouin R, Germain L
The Journal of biological chemistry 2005 Apr 1;280(13):12732-41
The Journal of biological chemistry 2005 Apr 1;280(13):12732-41
Cell-type-specific responses to chemotherapeutics in breast cancer.
Troester MA, Hoadley KA, Sørlie T, Herbert BS, Børresen-Dale AL, Lønning PE, Shay JW, Kaufmann WK, Perou CM
Cancer research 2004 Jun 15;64(12):4218-26
Cancer research 2004 Jun 15;64(12):4218-26
Role for BRG1 in cell cycle control and tumor suppression.
Hendricks KB, Shanahan F, Lees E
Molecular and cellular biology 2004 Jan;24(1):362-76
Molecular and cellular biology 2004 Jan;24(1):362-76
Functional and physical interaction of the human ARF tumor suppressor with Tat-binding protein-1.
Pollice A, Nasti V, Ronca R, Vivo M, Lo Iacono M, Calogero R, Calabrò V, La Mantia G
The Journal of biological chemistry 2004 Feb 20;279(8):6345-53
The Journal of biological chemistry 2004 Feb 20;279(8):6345-53
P27Kip1 and p21Cip1 are not required for the formation of active D cyclin-cdk4 complexes.
Bagui TK, Mohapatra S, Haura E, Pledger WJ
Molecular and cellular biology 2003 Oct;23(20):7285-90
Molecular and cellular biology 2003 Oct;23(20):7285-90
Caffeine and human DNA metabolism: the magic and the mystery.
Kaufmann WK, Heffernan TP, Beaulieu LM, Doherty S, Frank AR, Zhou Y, Bryant MF, Zhou T, Luche DD, Nikolaishvili-Feinberg N, Simpson DA, Cordeiro-Stone M
Mutation research 2003 Nov 27;532(1-2):85-102
Mutation research 2003 Nov 27;532(1-2):85-102
S100C/A11 is a key mediator of Ca(2+)-induced growth inhibition of human epidermal keratinocytes.
Sakaguchi M, Miyazaki M, Takaishi M, Sakaguchi Y, Makino E, Kataoka N, Yamada H, Namba M, Huh NH
The Journal of cell biology 2003 Nov 24;163(4):825-35
The Journal of cell biology 2003 Nov 24;163(4):825-35
Mammary epithelial cells of PR-A transgenic mice exhibit distinct alterations in gene expression and growth potential associated with transformation.
Chou YC, Uehara N, Lowry JR, Shyamala G
Carcinogenesis 2003 Mar;24(3):403-9
Carcinogenesis 2003 Mar;24(3):403-9
Delineation of the molecular basis for selenium-induced growth arrest in human prostate cancer cells by oligonucleotide array.
Dong Y, Zhang H, Hawthorn L, Ganther HE, Ip C
Cancer research 2003 Jan 1;63(1):52-9
Cancer research 2003 Jan 1;63(1):52-9
MDM2 promotes p21waf1/cip1 proteasomal turnover independently of ubiquitylation.
Jin Y, Lee H, Zeng SX, Dai MS, Lu H
The EMBO journal 2003 Dec 1;22(23):6365-77
The EMBO journal 2003 Dec 1;22(23):6365-77
G(1) and G(2) cell-cycle arrest following microtubule depolymerization in human breast cancer cells.
Blajeski AL, Phan VA, Kottke TJ, Kaufmann SH
The Journal of clinical investigation 2002 Jul;110(1):91-9
The Journal of clinical investigation 2002 Jul;110(1):91-9
E2F4/5 and p107 as Smad cofactors linking the TGFbeta receptor to c-myc repression.
Chen CR, Kang Y, Siegel PM, Massagué J
Cell 2002 Jul 12;110(1):19-32
Cell 2002 Jul 12;110(1):19-32
Differential roles of p21(Waf1) and p27(Kip1) in modulating chemosensitivity and their possible application in drug discovery studies.
Schmidt M, Lu Y, Parant JM, Lozano G, Bacher G, Beckers T, Fan Z
Molecular pharmacology 2001 Nov;60(5):900-6
Molecular pharmacology 2001 Nov;60(5):900-6
Notch signaling induces cell cycle arrest in small cell lung cancer cells.
Sriuranpong V, Borges MW, Ravi RK, Arnold DR, Nelkin BD, Baylin SB, Ball DW
Cancer research 2001 Apr 1;61(7):3200-5
Cancer research 2001 Apr 1;61(7):3200-5
Comparison of potential markers of farnesyltransferase inhibition.
Adjei AA, Davis JN, Erlichman C, Svingen PA, Kaufmann SH
Clinical cancer research : an official journal of the American Association for Cancer Research 2000 Jun;6(6):2318-25
Clinical cancer research : an official journal of the American Association for Cancer Research 2000 Jun;6(6):2318-25
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Supportive validation
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- Western blot of p21WAF11 using p21WAF11 Monoclonal Antibody (MA5-14350) on SW80 Cells and PC12 Cells.
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- HeLa cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus CDKN1A siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.
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- Formalin-fixed, paraffin-embedded human colon cancer stained with p21 antibody1 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
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Supportive validation
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- Flow cytometry analysis of p21WAF11 in Hela cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p21WAF11 monoclonal antibody (Product # MA5-14353) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
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- Biotin
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- Flow cytometry analysis of p21WAF11 in MCF-7 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/ml, fixed with 2% paraformaldehyde and washed with PBS. Cells were penetrated by dropping the supernatant, adding 90% methanol and incubated for 10 minutes at room temperature. Follwing penetration, cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a p21WAF11 monoclonal antibody (Product # MA5-14353) at a dilution of 2 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
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- Biotin
Supportive validation
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- Immunoprecipitation of p21WAF11 using p21WAF11 Monoclonal Antibody (MA5-14350) on Native Human LS174T Cells.
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- Immunofluorescent analysis of p21WAF11 (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p21WAF11 monoclonal antibody (Product # MA5-14353) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Conjugate
- Biotin
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of p21WAF11 (green) showing staining in the cytoplasm and nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p21WAF11 monoclonal antibody (Product # MA5-14353) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
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- Biotin
