MA5-14353

antibody from Invitrogen Antibodies

Targeting: CDKN1A CAP20, CDKN1, CIP1, P21, p21CIP1, p21Cip1/Waf1, SDI1, WAF1

Immunocytochemistry

Immunoprecipitation

Antibody data

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Validation data

Product number: MA5-14353 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-p21 Monoclonal Antibody (CP74)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-14353 targets p21WAF11 in FACS, IHC (P), IP, ICC/IF and WB applications and shows reactivity with Human, Mouse and Rat samples. The MA5-14353 immunogen is full length human recombinant p21 protein.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: CP74
Vial size: 500 µL
Concentration: 0.4 mg/mL
Storage: 4° C

CDKN1A protein structure - MA5-14353 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of p21WAF11 using p21WAF11 Monoclonal Antibody (MA5-14353) on Native Human LS174T Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of p21WAF11 (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p21WAF11 monoclonal antibody (Product # MA5-14353) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of p21WAF11 (green) showing staining in the cytoplasm and nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a p21WAF11 monoclonal antibody (Product # MA5-14353) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.