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ELISAAntibody data
- Antibody Data
- Antigen structure
- References [38]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- 10048-2-Ig - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10048-2-Ig, RRID:AB_2177320
- Product name
- RB1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated RB1 antibody (Cat. #10048-2-Ig) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references E7-Targeted Nanotherapeutics for Key HPV Afflicted Cervical Lesions by Employing CRISPR/Cas9 and Poly (Beta-Amino Ester).
SIRT1 Deacetylates TET2 and Promotes Its Ubiquitination Degradation to Achieve Neuroprotection Against Parkinson's Disease.
Th17/IL-17 induces endothelial cell senescence via activation of NF-κB/p53/Rb signaling pathway.
Knockout of mafba Causes Inner-Ear Developmental Defects in Zebrafish via the Impairment of Proliferation and Differentiation of Ionocyte Progenitor Cells.
The LncRNA RP11-301G19.1/miR-582-5p/HMGB2 axis modulates the proliferation and apoptosis of multiple myeloma cancer cells via the PI3K/AKT signalling pathway.
(20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells.
The application of CRISPR/Cas9 system in cervical carcinogenesis.
ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.
Molecular targeting of vulnerable RNA sequences in SARS CoV-2: identifying clinical feasibility.
In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system.
Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms.
FAIM-S functions as a negative regulator of NF-κB pathway and blocks cell cycle progression in NSCLC cells.
Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.
Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.
A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.
Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway.
Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.
Nanoparticles Based on Poly (β-Amino Ester) and HPV16-Targeting CRISPR/shRNA as Potential Drugs for HPV16-Related Cervical Malignancy.
CDKN2B deletion is essential for pancreatic cancer development instead of unmeaningful co-deletion due to juxtaposition to CDKN2A.
PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.
HOXD-AS1 functions as an oncogenic ceRNA to promote NSCLC cell progression by sequestering miR-147a.
The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.
Simvastatin suppresses the DNA replication licensing factor MCM7 and inhibits the growth of tamoxifen-resistant breast cancer cells.
TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.
Palbociclib inhibits epithelial-mesenchymal transition and metastasis in breast cancer via c-Jun/COX-2 signaling pathway.
Down-regulation of miR-675-5p contributes to tumor progression and development by targeting pro-tumorigenic GPR55 in non-small cell lung cancer.
Poldip2 knockout results in perinatal lethality, reduced cellular growth and increased autophagy of mouse embryonic fibroblasts.
Ginsenoside Rg3 inhibits melanoma cell proliferation through down-regulation of histone deacetylase 3 (HDAC3) and increase of p53 acetylation.
Disruption of HPV16-E7 by CRISPR/Cas system induces apoptosis and growth inhibition in HPV16 positive human cervical cancer cells.
Zinc finger nucleases targeting the human papillomavirus E7 oncogene induce E7 disruption and a transformed phenotype in HPV16/18-positive cervical cancer cells.
miR-375 activates p21 and suppresses telomerase activity by coordinately regulating HPV E6/E7, E6AP, CIP2A, and 14-3-3ζ.
Zbtb7a suppresses prostate cancer through repression of a Sox9-dependent pathway for cellular senescence bypass and tumor invasion.
Identification and functional analysis of 9p24 amplified genes in human breast cancer.
Betulinic acid decreases specificity protein 1 (Sp1) level via increasing the sumoylation of sp1 to inhibit lung cancer growth.
Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene.
AMP-activated protein kinase is involved in neural stem cell growth suppression and cell cycle arrest by 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside and glucose deprivation by down-regulating phospho-retinoblastoma protein and cyclin D.
Downregulation of CCND1 and CDK6 by miR-34a induces cell cycle arrest.
Effect of exogenous p16ink4a and hRb1 genes on cell cycle regulation of osteosarcoma cell.
Xiong J, Tan S, Yu L, Shen H, Qu S, Zhang C, Ren C, Zhu D, Wang H
International journal of nanomedicine 2021;16:7609-7622
International journal of nanomedicine 2021;16:7609-7622
SIRT1 Deacetylates TET2 and Promotes Its Ubiquitination Degradation to Achieve Neuroprotection Against Parkinson's Disease.
Li X, Liu T, Wu TT, Feng Y, Peng SJ, Yin H, Wu YC
Frontiers in neurology 2021;12:652882
Frontiers in neurology 2021;12:652882
Th17/IL-17 induces endothelial cell senescence via activation of NF-κB/p53/Rb signaling pathway.
Zhang L, Liu M, Liu W, Hu C, Li H, Deng J, Cao Q, Wang Y, Hu W, Li Q
Laboratory investigation; a journal of technical methods and pathology 2021 Nov;101(11):1418-1426
Laboratory investigation; a journal of technical methods and pathology 2021 Nov;101(11):1418-1426
Knockout of mafba Causes Inner-Ear Developmental Defects in Zebrafish via the Impairment of Proliferation and Differentiation of Ionocyte Progenitor Cells.
Chen X, Huang Y, Gao P, Lv Y, Jia D, Sun K, Han Y, Hu H, Tang Z, Ren X, Liu M
Biomedicines 2021 Nov 16;9(11)
Biomedicines 2021 Nov 16;9(11)
The LncRNA RP11-301G19.1/miR-582-5p/HMGB2 axis modulates the proliferation and apoptosis of multiple myeloma cancer cells via the PI3K/AKT signalling pathway.
Wang F, Luo Y, Zhang L, Younis M, Yuan L
Cancer gene therapy 2021 Mar 11;
Cancer gene therapy 2021 Mar 11;
(20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells.
Chen C, Wang YS, Zhang ET, Li GA, Liu WY, Li Y, Jin YH
International journal of molecular sciences 2021 Dec 6;22(23)
International journal of molecular sciences 2021 Dec 6;22(23)
The application of CRISPR/Cas9 system in cervical carcinogenesis.
Gao C, Wu P, Yu L, Liu L, Liu H, Tan X, Wang L, Huang X, Wang H
Cancer gene therapy 2021 Aug 4;
Cancer gene therapy 2021 Aug 4;
ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration.
Ding J, Zhang R, Li H, Ji Q, Cheng X, Thorne RF, Hondermarck H, Liu X, Shen C
Aging 2021 Apr 6;13(7):10703-10723
Aging 2021 Apr 6;13(7):10703-10723
Molecular targeting of vulnerable RNA sequences in SARS CoV-2: identifying clinical feasibility.
Gallicano GI, Casey JL, Fu J, Mahapatra S
Gene therapy 2020 Nov 12;
Gene therapy 2020 Nov 12;
In vitro and in vivo growth inhibition of human cervical cancer cells via human papillomavirus E6/E7 mRNAs' cleavage by CRISPR/Cas13a system.
Chen Y, Jiang H, Wang T, He D, Tian R, Cui Z, Tian X, Gao Q, Ma X, Yang J, Wu J, Tan S, Xu H, Tang X, Wang Y, Yu Z, Han H, Das BC, Severinov K, Hitzeroth II, Debata PR, Xu W, Fan W, Jin Z, Cao C, Yu M, Xie W, Huang Z, Hu Z, You Z
Antiviral research 2020 Jun;178:104794
Antiviral research 2020 Jun;178:104794
Ribociclib mitigates cisplatin-associated kidney injury through retinoblastoma-1 dependent mechanisms.
Kim JY, Jayne LA, Bai Y, Feng MJHH, Clark MA, Chung S, W Christman J, Cianciolo RE, Pabla NS
Biochemical pharmacology 2020 Jul;177:113939
Biochemical pharmacology 2020 Jul;177:113939
FAIM-S functions as a negative regulator of NF-κB pathway and blocks cell cycle progression in NSCLC cells.
Wang P, Xun W, Han T, Cheng Z
Cell cycle (Georgetown, Tex.) 2020 Dec;19(24):3458-3467
Cell cycle (Georgetown, Tex.) 2020 Dec;19(24):3458-3467
Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.
Huang S, Wang LL, Xue NN, Li C, Guo HH, Ren TK, Zhan Y, Li WB, Zhang J, Chen XG, Han YX, Zhang JL, Jiang JD
Theranostics 2019;9(23):6745-6763
Theranostics 2019;9(23):6745-6763
Intestinal PPARα Protects Against Colon Carcinogenesis via Regulation of Methyltransferases DNMT1 and PRMT6.
Luo Y, Xie C, Brocker CN, Fan J, Wu X, Feng L, Wang Q, Zhao J, Lu D, Tandon M, Cam M, Krausz KW, Liu W, Gonzalez FJ
Gastroenterology 2019 Sep;157(3):744-759.e4
Gastroenterology 2019 Sep;157(3):744-759.e4
A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer.
Wang Y, Han T, Gan M, Guo M, Xie C, Jin J, Zhang S, Wang P, Cao J, Wang JB
Cell cycle (Georgetown, Tex.) 2019 May;18(9):1019-1032
Cell cycle (Georgetown, Tex.) 2019 May;18(9):1019-1032
Ribociclib (LEE011) suppresses cell proliferation and induces apoptosis of MDA-MB-231 by inhibiting CDK4/6-cyclin D-Rb-E2F pathway.
Li T, Xiong Y, Wang Q, Chen F, Zeng Y, Yu X, Wang Y, Zhou F, Zhou Y
Artificial cells, nanomedicine, and biotechnology 2019 Dec;47(1):4001-4011
Artificial cells, nanomedicine, and biotechnology 2019 Dec;47(1):4001-4011
Ribociclib, a selective cyclin D kinase 4/6 inhibitor, inhibits proliferation and induces apoptosis of human cervical cancer in vitro and in vivo.
Xiong Y, Li T, Assani G, Ling H, Zhou Q, Zeng Y, Zhou F, Zhou Y
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Apr;112:108602
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2019 Apr;112:108602
Nanoparticles Based on Poly (β-Amino Ester) and HPV16-Targeting CRISPR/shRNA as Potential Drugs for HPV16-Related Cervical Malignancy.
Zhu D, Shen H, Tan S, Hu Z, Wang L, Yu L, Tian X, Ding W, Ren C, Gao C, Cheng J, Deng M, Liu R, Hu J, Xi L, Wu P, Zhang Z, Ma D, Wang H
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Oct 3;26(10):2443-2455
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Oct 3;26(10):2443-2455
CDKN2B deletion is essential for pancreatic cancer development instead of unmeaningful co-deletion due to juxtaposition to CDKN2A.
Tu Q, Hao J, Zhou X, Yan L, Dai H, Sun B, Yang D, An S, Lv L, Jiao B, Chen C, Lai R, Shi P, Zhao X
Oncogene 2018 Jan 4;37(1):128-138
Oncogene 2018 Jan 4;37(1):128-138
PTBP3 splicing factor promotes hepatocellular carcinoma by destroying the splicing balance of NEAT1 and pre-miR-612.
Yang X, Qu S, Wang L, Zhang H, Yang Z, Wang J, Dai B, Tao K, Shang R, Liu Z, Li X, Zhang Z, Xia C, Ma B, Liu W, Li H, Dou K
Oncogene 2018 Dec;37(50):6399-6413
Oncogene 2018 Dec;37(50):6399-6413
HOXD-AS1 functions as an oncogenic ceRNA to promote NSCLC cell progression by sequestering miR-147a.
Wang Q, Jiang S, Song A, Hou S, Wu Q, Qi L, Gao X
OncoTargets and therapy 2017;10:4753-4763
OncoTargets and therapy 2017;10:4753-4763
The synthetic antihyperlipidemic drug potassium piperate selectively kills breast cancer cells through inhibiting G1-S-phase transition and inducing apoptosis.
Fan L, Cao X, Yan H, Wang Q, Tian X, Zhang L, He X, Borjihan G, Morigen
Oncotarget 2017 Jul 18;8(29):47250-47268
Oncotarget 2017 Jul 18;8(29):47250-47268
Simvastatin suppresses the DNA replication licensing factor MCM7 and inhibits the growth of tamoxifen-resistant breast cancer cells.
Liang Z, Li W, Liu J, Li J, He F, Jiang Y, Yang L, Li P, Wang B, Wang Y, Ren Y, Yang J, Luo Z, Vaziri C, Liu P
Scientific reports 2017 Feb 2;7:41776
Scientific reports 2017 Feb 2;7:41776
TALEN-mediated targeting of HPV oncogenes ameliorates HPV-related cervical malignancy.
Hu Z, Ding W, Zhu D, Yu L, Jiang X, Wang X, Zhang C, Wang L, Ji T, Liu D, He D, Xia X, Zhu T, Wei J, Wu P, Wang C, Xi L, Gao Q, Chen G, Liu R, Li K, Li S, Wang S, Zhou J, Ma D, Wang H
The Journal of clinical investigation 2015 Jan;125(1):425-36
The Journal of clinical investigation 2015 Jan;125(1):425-36
Palbociclib inhibits epithelial-mesenchymal transition and metastasis in breast cancer via c-Jun/COX-2 signaling pathway.
Qin G, Xu F, Qin T, Zheng Q, Shi D, Xia W, Tian Y, Tang Y, Wang J, Xiao X, Deng W, Wang S
Oncotarget 2015 Dec 8;6(39):41794-808
Oncotarget 2015 Dec 8;6(39):41794-808
Down-regulation of miR-675-5p contributes to tumor progression and development by targeting pro-tumorigenic GPR55 in non-small cell lung cancer.
He D, Wang J, Zhang C, Shan B, Deng X, Li B, Zhou Y, Chen W, Hong J, Gao Y, Chen Z, Duan C
Molecular cancer 2015 Apr 1;14:73
Molecular cancer 2015 Apr 1;14:73
Poldip2 knockout results in perinatal lethality, reduced cellular growth and increased autophagy of mouse embryonic fibroblasts.
Brown DI, Lassègue B, Lee M, Zafari R, Long JS, Saavedra HI, Griendling KK
PloS one 2014;9(5):e96657
PloS one 2014;9(5):e96657
Ginsenoside Rg3 inhibits melanoma cell proliferation through down-regulation of histone deacetylase 3 (HDAC3) and increase of p53 acetylation.
Shan X, Fu YS, Aziz F, Wang XQ, Yan Q, Liu JW
PloS one 2014;9(12):e115401
PloS one 2014;9(12):e115401
Disruption of HPV16-E7 by CRISPR/Cas system induces apoptosis and growth inhibition in HPV16 positive human cervical cancer cells.
Hu Z, Yu L, Zhu D, Ding W, Wang X, Zhang C, Wang L, Jiang X, Shen H, He D, Li K, Xi L, Ma D, Wang H
BioMed research international 2014;2014:612823
BioMed research international 2014;2014:612823
Zinc finger nucleases targeting the human papillomavirus E7 oncogene induce E7 disruption and a transformed phenotype in HPV16/18-positive cervical cancer cells.
Ding W, Hu Z, Zhu D, Jiang X, Yu L, Wang X, Zhang C, Wang L, Ji T, Li K, He D, Xia X, Liu D, Zhou J, Ma D, Wang H
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Dec 15;20(24):6495-503
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Dec 15;20(24):6495-503
miR-375 activates p21 and suppresses telomerase activity by coordinately regulating HPV E6/E7, E6AP, CIP2A, and 14-3-3ζ.
Jung HM, Phillips BL, Chan EK
Molecular cancer 2014 Apr 8;13:80
Molecular cancer 2014 Apr 8;13:80
Zbtb7a suppresses prostate cancer through repression of a Sox9-dependent pathway for cellular senescence bypass and tumor invasion.
Wang G, Lunardi A, Zhang J, Chen Z, Ala U, Webster KA, Tay Y, Gonzalez-Billalabeitia E, Egia A, Shaffer DR, Carver B, Liu XS, Taulli R, Kuo WP, Nardella C, Signoretti S, Cordon-Cardo C, Gerald WL, Pandolfi PP
Nature genetics 2013 Jul;45(7):739-746
Nature genetics 2013 Jul;45(7):739-746
Identification and functional analysis of 9p24 amplified genes in human breast cancer.
Wu J, Liu S, Liu G, Dombkowski A, Abrams J, Martin-Trevino R, Wicha MS, Ethier SP, Yang ZQ
Oncogene 2012 Jan 19;31(3):333-41
Oncogene 2012 Jan 19;31(3):333-41
Betulinic acid decreases specificity protein 1 (Sp1) level via increasing the sumoylation of sp1 to inhibit lung cancer growth.
Hsu TI, Wang MC, Chen SY, Huang ST, Yeh YM, Su WC, Chang WC, Hung JJ
Molecular pharmacology 2012 Dec;82(6):1115-28
Molecular pharmacology 2012 Dec;82(6):1115-28
Putative tumor suppressor miR-145 inhibits colon cancer cell growth by targeting oncogene Friend leukemia virus integration 1 gene.
Zhang J, Guo H, Zhang H, Wang H, Qian G, Fan X, Hoffman AR, Hu JF, Ge S
Cancer 2011 Jan 1;117(1):86-95
Cancer 2011 Jan 1;117(1):86-95
AMP-activated protein kinase is involved in neural stem cell growth suppression and cell cycle arrest by 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside and glucose deprivation by down-regulating phospho-retinoblastoma protein and cyclin D.
Zang Y, Yu LF, Nan FJ, Feng LY, Li J
The Journal of biological chemistry 2009 Mar 6;284(10):6175-84
The Journal of biological chemistry 2009 Mar 6;284(10):6175-84
Downregulation of CCND1 and CDK6 by miR-34a induces cell cycle arrest.
Sun F, Fu H, Liu Q, Tie Y, Zhu J, Xing R, Sun Z, Zheng X
FEBS letters 2008 Apr 30;582(10):1564-8
FEBS letters 2008 Apr 30;582(10):1564-8
Effect of exogenous p16ink4a and hRb1 genes on cell cycle regulation of osteosarcoma cell.
Liao X, Yang S, Shao Z, Li J, Liu Y, Xiong X, Liu X
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2005;25(6):679-82
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2005;25(6):679-82
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 10048-2-Ig(RB1 antibody) at dilution of 1:3000
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-RB1 (IP:10048-2-Ig, 3ug; Detection:10048-2-Ig 1:500) with A431 cells lysate 3000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The RB1 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human RB1. This antibody recognizes human, mouse antigen. The RB1 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
