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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AP3236a - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AP3236a, RRID:AB_637399
- Product name
- Phospho-RB(S612) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.38 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1.
Rizzolio F, Lucchetti C, Caligiuri I, Marchesi I, Caputo M, Klein-Szanto AJ, Bagella L, Castronovo M, Giordano A
Cell death and differentiation 2012 Jul;19(7):1152-61
Cell death and differentiation 2012 Jul;19(7):1152-61
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "The anti-RB Pab (Cat. #AP3236a) is used in Western blot to detect RB in, from left to right, HL60, T47D, and SK-BR-3 tissue lysates."
- Primary Ab dilution
- 1:1000
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma"
- Primary Ab dilution
- 1:50~100
