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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX100545 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100545, RRID:AB_11163170
- Product name
- Rb antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
The synthetic flavonoid WYC02-9 inhibits cervical cancer cell migration/invasion and angiogenesis via MAPK14 signaling.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
The synthetic flavonoid WYC02-9 inhibits cervical cancer cell migration/invasion and angiogenesis via MAPK14 signaling.
Chen YJ, Cheng YJ, Hung AC, Wu YC, Hou MF, Tyan YC, Yuan SS
Gynecologic oncology 2013 Dec;131(3):734-43
Gynecologic oncology 2013 Dec;131(3):734-43
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: U2OS 5% SDS PAGE GTX100545 diluted at 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rb antibody detects Rb protein by western blot analysis.A. 30 ?g A431 whole cell lysate/extract5% SDS-PAGERb antibody (GTX100545) dilution: 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rb antibody detects Rb protein at nucleus by immunofluorescent analysis.Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Rb protein stained by Rb antibody (GTX100545) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rb antibody immunoprecipitates retinoblastoma 1 protein in IP experiments.IP samples: HeLa whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of retinoblastoma 1 protein by 4 £gg Rb antibody (GTX100545)7.5 % SDS-PAGEThe immunoprecipitated retinoblastoma 1 protein was detected by Rb antibody (GTX100545) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rb antibody detects Rb protein at nucleus on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. Rb antibody (GTX100545) dilution: 1:500.
