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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [23]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- V3218 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Retinoblastoma / Rb Antibody
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 1F8
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- The Rb antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Submitted references Genomewide copy number analysis of Müllerian adenosarcoma identified chromosomal instability in the aggressive subgroup.
Follicular dendritic cell sarcoma: clinicopathologic study of 15 cases with emphasis on novel expression of MDM2, somatostatin receptor 2A, and PD-L1.
CDK 4/6 inhibitor palbociclib (PD0332991) in Rb+ advanced breast cancer: phase II activity, safety, and predictive biomarker assessment.
Selective impact of CDK4/6 suppression on patient-derived models of pancreatic cancer.
Mass-array screening of frequent mutations in cancers reveals RB1 alterations in aggressive adrenocortical carcinomas.
The retinoblastoma tumor suppressor pathway modulates the invasiveness of ErbB2-positive breast cancer.
RB1 gene in Merkel cell carcinoma: hypermethylation in all tumors and concurrent heterozygous deletions in the polyomavirus-negative subgroup.
Identification of oropharyngeal squamous cell carcinomas with active HPV16 involvement by immunohistochemical analysis of the retinoblastoma protein pathway.
Human papilloma virus genome is rare in North American non-small cell lung carcinoma patients.
Impact of Hepatitis C Virus (HCV) infection on biomolecular markers influencing the pathogenesis of bladder cancer.
Biological activity of probable/possible high-risk human papillomavirus types in cervical cancer.
Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7.
Alteration of G1/S transition regulators influences recurrences in head and neck squamous carcinomas.
Retinoblastoma and phosphate and tensin homolog tumor suppressors: impact on ductal carcinoma in situ progression.
Blockade of phospholipid scramblase 1 with its N-terminal domain antibody reduces tumorigenesis of colorectal carcinomas in vitro and in vivo.
HFE polymorphisms influence the response to chemotherapeutic agents via induction of p16INK4A.
Therapeutically activating RB: reestablishing cell cycle control in endocrine therapy-resistant breast cancer.
Rare MDM4 gene amplification in colorectal cancer: The principle of a mutually exclusive relationship between MDM alteration and TP53 inactivation is not applicable.
Simultaneous aberrations of single CDKN2A network components and a high Rb phosphorylation status can differentiate subgroups of primary cutaneous B-cell lymphomas.
Use of tissue microarray to facilitate oncology research.
Linkage of the potent leukemogenic activity of Meis1 to cell-cycle entry and transcriptional regulation of cyclin D3.
Activation of Wnt signaling pathway by human papillomavirus E6 and E7 oncogenes in HPV16-positive oropharyngeal squamous carcinoma cells.
Inducible activation of IFI 16 results in suppression of telomerase activity, growth suppression and induction of cellular senescence.
Lee JC, Lu TP, Changou CA, Liang CW, Huang HN, Lauria A, Huang HY, Lin CY, Chiang YC, Davidson B, Lin MC, Kuo KT
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2016 Sep;29(9):1070-82
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2016 Sep;29(9):1070-82
Follicular dendritic cell sarcoma: clinicopathologic study of 15 cases with emphasis on novel expression of MDM2, somatostatin receptor 2A, and PD-L1.
Agaimy A, Michal M, Hadravsky L, Michal M
Annals of diagnostic pathology 2016 Aug;23:21-8
Annals of diagnostic pathology 2016 Aug;23:21-8
CDK 4/6 inhibitor palbociclib (PD0332991) in Rb+ advanced breast cancer: phase II activity, safety, and predictive biomarker assessment.
DeMichele A, Clark AS, Tan KS, Heitjan DF, Gramlich K, Gallagher M, Lal P, Feldman M, Zhang P, Colameco C, Lewis D, Langer M, Goodman N, Domchek S, Gogineni K, Rosen M, Fox K, O'Dwyer P
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Mar 1;21(5):995-1001
Clinical cancer research : an official journal of the American Association for Cancer Research 2015 Mar 1;21(5):995-1001
Selective impact of CDK4/6 suppression on patient-derived models of pancreatic cancer.
Witkiewicz AK, Borja NA, Franco J, Brody JR, Yeo CJ, Mansour J, Choti MA, McCue P, Knudsen ES
Oncotarget 2015 Jun 30;6(18):15788-801
Oncotarget 2015 Jun 30;6(18):15788-801
Mass-array screening of frequent mutations in cancers reveals RB1 alterations in aggressive adrenocortical carcinomas.
Ragazzon B, Libé R, Assié G, Tissier F, Barreau O, Houdayer C, Perlemoine K, Audebourg A, Clauser E, René-Corail F, Bertagna X, Dousset B, Bertherat J, Groussin L
European journal of endocrinology 2014 Mar;170(3):385-91
European journal of endocrinology 2014 Mar;170(3):385-91
The retinoblastoma tumor suppressor pathway modulates the invasiveness of ErbB2-positive breast cancer.
Witkiewicz AK, Cox DW, Rivadeneira D, Ertel AE, Fortina P, Schwartz GF, Knudsen ES
Oncogene 2014 Jul 24;33(30):3980-91
Oncogene 2014 Jul 24;33(30):3980-91
RB1 gene in Merkel cell carcinoma: hypermethylation in all tumors and concurrent heterozygous deletions in the polyomavirus-negative subgroup.
Sahi H, Savola S, Sihto H, Koljonen V, Bohling T, Knuutila S
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2014 Dec;122(12):1157-66
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2014 Dec;122(12):1157-66
Identification of oropharyngeal squamous cell carcinomas with active HPV16 involvement by immunohistochemical analysis of the retinoblastoma protein pathway.
Holzinger D, Flechtenmacher C, Henfling N, Kaden I, Grabe N, Lahrmann B, Schmitt M, Hess J, Pawlita M, Bosch FX
International journal of cancer 2013 Sep 15;133(6):1389-99
International journal of cancer 2013 Sep 15;133(6):1389-99
Human papilloma virus genome is rare in North American non-small cell lung carcinoma patients.
Yanagawa N, Wang A, Kohler D, Santos Gda C, Sykes J, Xu J, Pintilie M, Tsao MS
Lung cancer (Amsterdam, Netherlands) 2013 Mar;79(3):215-20
Lung cancer (Amsterdam, Netherlands) 2013 Mar;79(3):215-20
Impact of Hepatitis C Virus (HCV) infection on biomolecular markers influencing the pathogenesis of bladder cancer.
Hemmaid KZ, Awadalla A, Elsawy E, Hussein AA, Abdel-Aziz A, Shokeir AA, El-Hefnawy AS, Abol-Enein H
Infectious agents and cancer 2013 Jun 28;8(1):24
Infectious agents and cancer 2013 Jun 28;8(1):24
Biological activity of probable/possible high-risk human papillomavirus types in cervical cancer.
Halec G, Schmitt M, Dondog B, Sharkhuu E, Wentzensen N, Gheit T, Tommasino M, Kommoss F, Bosch FX, Franceschi S, Clifford G, Gissmann L, Pawlita M
International journal of cancer 2013 Jan 1;132(1):63-71
International journal of cancer 2013 Jan 1;132(1):63-71
Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7.
Henken FE, Oosterhuis K, Öhlschläger P, Bosch L, Hooijberg E, Haanen JB, Steenbergen RD
Vaccine 2012 Jun 13;30(28):4259-66
Vaccine 2012 Jun 13;30(28):4259-66
Alteration of G1/S transition regulators influences recurrences in head and neck squamous carcinomas.
Canzonieri V, Barzan L, Franchin G, Vaccher E, Talamini R, Sulfaro S, Baldassarre G
Journal of cellular physiology 2012 Jan;227(1):233-8
Journal of cellular physiology 2012 Jan;227(1):233-8
Retinoblastoma and phosphate and tensin homolog tumor suppressors: impact on ductal carcinoma in situ progression.
Knudsen ES, Pajak TF, Qeenan M, McClendon AK, Armon BD, Schwartz GF, Witkiewicz AK
Journal of the National Cancer Institute 2012 Dec 5;104(23):1825-36
Journal of the National Cancer Institute 2012 Dec 5;104(23):1825-36
Blockade of phospholipid scramblase 1 with its N-terminal domain antibody reduces tumorigenesis of colorectal carcinomas in vitro and in vivo.
Fan CW, Chen CY, Chen KT, Shen CR, Kuo YB, Chen YS, Chou YP, Wei WS, Chan EC
Journal of translational medicine 2012 Dec 24;10:254
Journal of translational medicine 2012 Dec 24;10:254
HFE polymorphisms influence the response to chemotherapeutic agents via induction of p16INK4A.
Lee SY, Liu S, Mitchell RM, Slagle-Webb B, Hong YS, Sheehan JM, Connor JR
International journal of cancer 2011 Nov 1;129(9):2104-14
International journal of cancer 2011 Nov 1;129(9):2104-14
Therapeutically activating RB: reestablishing cell cycle control in endocrine therapy-resistant breast cancer.
Thangavel C, Dean JL, Ertel A, Knudsen KE, Aldaz CM, Witkiewicz AK, Clarke R, Knudsen ES
Endocrine-related cancer 2011 Jun;18(3):333-45
Endocrine-related cancer 2011 Jun;18(3):333-45
Rare MDM4 gene amplification in colorectal cancer: The principle of a mutually exclusive relationship between MDM alteration and TP53 inactivation is not applicable.
Suda T, Yoshihara M, Nakamura Y, Sekiguchi H, Godai TI, Sugano N, Tsuchida K, Shiozawa M, Sakuma Y, Tsuchiya E, Kameda Y, Akaike M, Matsukuma S, Miyagi Y
Oncology reports 2011 Jul;26(1):49-54
Oncology reports 2011 Jul;26(1):49-54
Simultaneous aberrations of single CDKN2A network components and a high Rb phosphorylation status can differentiate subgroups of primary cutaneous B-cell lymphomas.
Kaune KM, Neumann C, Hallermann C, Haller F, Schön MP, Middel P
Experimental dermatology 2011 Apr;20(4):331-5
Experimental dermatology 2011 Apr;20(4):331-5
Use of tissue microarray to facilitate oncology research.
Gouveris P, Weinberger PM, Psyrri A
Methods in molecular biology (Clifton, N.J.) 2010;632:239-50
Methods in molecular biology (Clifton, N.J.) 2010;632:239-50
Linkage of the potent leukemogenic activity of Meis1 to cell-cycle entry and transcriptional regulation of cyclin D3.
Argiropoulos B, Yung E, Xiang P, Lo CY, Kuchenbauer F, Palmqvist L, Reindl C, Heuser M, Sekulovic S, Rosten P, Muranyi A, Goh SL, Featherstone M, Humphries RK
Blood 2010 May 20;115(20):4071-82
Blood 2010 May 20;115(20):4071-82
Activation of Wnt signaling pathway by human papillomavirus E6 and E7 oncogenes in HPV16-positive oropharyngeal squamous carcinoma cells.
Rampias T, Boutati E, Pectasides E, Sasaki C, Kountourakis P, Weinberger P, Psyrri A
Molecular cancer research : MCR 2010 Mar;8(3):433-43
Molecular cancer research : MCR 2010 Mar;8(3):433-43
Inducible activation of IFI 16 results in suppression of telomerase activity, growth suppression and induction of cellular senescence.
Clarke CJ, Hii LL, Bolden JE, Johnstone RW
Journal of cellular biochemistry 2010 Jan 1;109(1):103-12
Journal of cellular biochemistry 2010 Jan 1;109(1):103-12
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A431 cell lysate (nuclear fraction) with Rb antibody (clone 1F8). Expected molecular weight ~106 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human colon with Rb antibody (clone 1F8). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
