P00039-1

antibody from Boster Biological Technology

Targeting: RB1 OSRC, PPP1R130, RB

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

P00039-1

Provider

Boster Biological Technology

Product name

Anti-Phospho-Rb (S807) RB1 Rabbit Monoclonal Antibody

Antibody type

Monoclonal

Description

Monoclonal antibody for CLEAVED RETINOBLASTOMA 1/RB1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat CLEAVED RETINOBLASTOMA 1/RB1 information: Molecular Weight: 106159 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Expressed in the retina.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

DGG-18

Vial size

100ug/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. Avoid repeated freezing and thawing.

References

Submitted references

Retarding the senescence of human vascular endothelial cells induced by hydrogen peroxide: effects of 17beta-estradiol (E2) mediated mitochondria protection.

Ruan Y, Wu S, Zhang L, Chen G, Lai W
Biogerontology 2014 Aug;15(4):367-75

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse spleen, using Phospho-Rb (S807) Antibody (P00039-1)RB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RB1 Antibody (P00039-1)overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image