MA5-11384

antibody from Invitrogen Antibodies

Targeting: RB1 OSRC, PPP1R130, RB

Provider product page for MA5-11384

Western blot

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [20]
Comments [0]
Validations
Western blot [2]
Immunocytochemistry [1]
Immunohistochemistry [1]
Flow cytometry [1]
Other assay [2]

Submit

Product number: MA5-11384 - Provider product page
Provider: Invitrogen Antibodies
Product name: Rb Monoclonal Antibody (1F8 (Rb1)), Biotin
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: MA5-11384 targets Retinoblastoma in FACS, ICC/IF, IHC (P), IP, and WB applications and shows reactivity with Human and Mouse samples. The MA5-11384 immunogen is recombinant human Rb protein.
Reactivity: Human, Mouse
Host: Mouse
Conjugate: Biotin
Isotype: IgG
Antibody clone number: 1F8 (Rb1)
Vial size: 500 µL
Concentration: 0.2 mg/mL
Storage: 4° C

RB1 protein structure - MA5-11384 shown in red.

Submitted references RB1 gene in Merkel cell carcinoma: hypermethylation in all tumors and concurrent heterozygous deletions in the polyomavirus-negative subgroup.

Sahi H, Savola S, Sihto H, Koljonen V, Bohling T, Knuutila S
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2014 Dec;122(12):1157-66

Human papilloma virus genome is rare in North American non-small cell lung carcinoma patients.

Yanagawa N, Wang A, Kohler D, Santos Gda C, Sykes J, Xu J, Pintilie M, Tsao MS
Lung cancer (Amsterdam, Netherlands) 2013 Mar;79(3):215-20

Alteration of G1/S transition regulators influences recurrences in head and neck squamous carcinomas.

Canzonieri V, Barzan L, Franchin G, Vaccher E, Talamini R, Sulfaro S, Baldassarre G
Journal of cellular physiology 2012 Jan;227(1):233-8

HFE polymorphisms influence the response to chemotherapeutic agents via induction of p16INK4A.

Lee SY, Liu S, Mitchell RM, Slagle-Webb B, Hong YS, Sheehan JM, Connor JR
International journal of cancer 2011 Nov 1;129(9):2104-14

Prognostic significance of the alterations of the G1-S checkpoint in localized leiomyosarcoma of the peripheral soft tissue.

Panelos J, Beltrami G, Scoccianti G, Capanna R, Paglierani M, Pepi M, Massi D, Franchi A
Annals of surgical oncology 2011 Feb;18(2):566-71

Loss of retinoblastoma tumor suppressor protein makes human breast cancer cells more sensitive to antimetabolite exposure.

Derenzini M, Donati G, Mazzini G, Montanaro L, Vici M, Ceccarelli C, Santini D, Taffurelli M, Treré D
Clinical cancer research : an official journal of the American Association for Cancer Research 2008 Apr 1;14(7):2199-209

Centrosome abnormalities in non-small cell lung cancer: correlations with DNA aneuploidy and expression of cell cycle regulatory proteins.

Jung CK, Jung JH, Lee KY, Kang CS, Kim M, Ko YH, Oh CS
Pathology, research and practice 2007;203(12):839-47

The inhibition of T-cells proliferation by mouse mesenchymal stem cells through the induction of p16INK4A-cyclin D1/cdk4 and p21waf1, p27kip1-cyclin E/cdk2 pathways.

Kim JA, Hong S, Lee B, Hong JW, Kwak JY, Cho S, Kim CC
Cellular immunology 2007 Jan;245(1):16-23

The expressions of the Rb pathway in cervical intraepithelial neoplasia; predictive and prognostic significance.

Nam EJ, Kim JW, Kim SW, Kim YT, Kim JH, Yoon BS, Cho NH, Kim S
Gynecologic oncology 2007 Jan;104(1):207-11

Different effects of ribosome biogenesis inhibition on cell proliferation in retinoblastoma protein- and p53-deficient and proficient human osteosarcoma cell lines.

Montanaro L, Mazzini G, Barbieri S, Vici M, Nardi-Pantoli A, Govoni M, Donati G, Treré D, Derenzini M
Cell proliferation 2007 Aug;40(4):532-49

Molecular and immunohistochemical analysis of the prognostic value of cell-cycle regulators in urothelial neoplasms of the bladder.

Yurakh AO, Ramos D, Calabuig-Fariñas S, López-Guerrero JA, Rubio J, Solsona E, Romanenko AM, Vozianov AF, Pellin A, Llombart-Bosch A
European urology 2006 Sep;50(3):506-15; discussion 515

Inhibitory effect of caffeic acid phenethyl ester on the growth of C6 glioma cells in vitro and in vivo.

Kuo HC, Kuo WH, Lee YJ, Lin WL, Chou FP, Tseng TH
Cancer letters 2006 Mar 28;234(2):199-208

Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells.

Choi JK, Murillo G, Su BN, Pezzuto JM, Kinghorn AD, Mehta RG
The FEBS journal 2006 Dec;273(24):5714-23

p53, p16 and cyclin D1: molecular determinants of radiotherapy treatment response in oral carcinoma.

Jayasurya R, Francis G, Kannan S, Lekshminarayanan K, Nalinakumari KR, Abraham T, Abraham EK, Nair MK
International journal of cancer 2004 May 1;109(5):710-6

The prevalence and clinicopathologic correlate of p16INK4a, retinoblastoma and p53 immunoreactivity in locally advanced urinary bladder cancer.

Tzai TS, Tsai YS, Chow NH
Urologic oncology 2004 Mar-Apr;22(2):112-8

The prognostic value of the AgNOR parameter in human breast cancer depends on the pRb and p53 status.

Derenzini M, Ceccarelli C, Santini D, Taffurelli M, Treré D
Journal of clinical pathology 2004 Jul;57(7):755-61

Nucleolar size and activity are related to pRb and p53 status in human breast cancer.

Treré D, Ceccarelli C, Montanaro L, Tosti E, Derenzini M
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2004 Dec;52(12):1601-7

Flavopiridol increases sensitization to gemcitabine in human gastrointestinal cancer cell lines and correlates with down-regulation of ribonucleotide reductase M2 subunit.

Jung CP, Motwani MV, Schwartz GK
Clinical cancer research : an official journal of the American Association for Cancer Research 2001 Aug;7(8):2527-36

p14ARF silencing by promoter hypermethylation mediates abnormal intracellular localization of MDM2.

Esteller M, Cordon-Cardo C, Corn PG, Meltzer SJ, Pohar KS, Watkins DN, Capella G, Peinado MA, Matias-Guiu X, Prat J, Baylin SB, Herman JG
Cancer research 2001 Apr 1;61(7):2816-21

Loss of p16INK4 expression by methylation is associated with lifespan extension of human mammary epithelial cells.

Huschtscha LI, Noble JR, Neumann AA, Moy EL, Barry P, Melki JR, Clark SJ, Reddel RR
Cancer research 1998 Aug 15;58(16):3508-12

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of Retinoblastoma using Retinoblastoma Monoclonal Antibody (Product # MA5-11384) on LS174T Cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Retinoblastoma was performed by loading 25 µg of K562 (Lane 1), B-3 (Lane 2) and rat eye (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a Retinoblastoma monoclonal antibody (Product # MA5-11387) at a dilution of 1:100 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 110 kDa in K562 and B-3 cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Retinoblastoma (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Retinoblastoma monoclonal antibody (Product # MA5-11387) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human breast carcinoma stained with Retinoblastoma antibody using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of Retinoblastoma in NIH/3T3 cells compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Retinoblastoma monoclonal antibody (Product # MA5-11387) at a dilution of 2 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Retinoblastoma using Retinoblastoma Monoclonal Antibody (Product # MA5-11384) on Native Human LS174T Cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Retinoblastoma using Retinoblastoma Monoclonal Antibody (Product # MA5-11384) on denatured Human HT29 Cells.
Conjugate: Biotin