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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-56598 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56598, RRID:AB_838783
- Product name
- Mouse Monoclonal RB1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The antibody recognizes cleaved Rb (DRb-p70). The antibody does not recognize full-length Rb. In camptothecin treated HL60, an ~68 kDa band is observed. DRb-p70 exists in vivo as an N-terminally truncated form of Rb (Liu et al.). DRb-p70 is thought to be the product of alternative translation and is expressed in primary myeloid cells in fetal liver, bone marrow and spleen. DRb-p70 is also specifically expressed in tumor-associated macrophages (TAMs). TAMs are an important targets of cancer therapy. DRb-p70 is a marker of TAMs, and maybe useful for identifying tumors with macrophage infiltration (Liu et al, 2005).
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The cell cycle factor E2F-1 activates Bnip3 and the intrinsic death pathway in ventricular myocytes.
A novel form of pRb expressed during normal myelopoiesis and in tumour-associated macrophages.
Yurkova N, Shaw J, Blackie K, Weidman D, Jayas R, Flynn B, Kirshenbaum LA
Circulation research 2008 Feb 29;102(4):472-9
Circulation research 2008 Feb 29;102(4):472-9
A novel form of pRb expressed during normal myelopoiesis and in tumour-associated macrophages.
Liu HP, Thompson AM, Macleod KF
Cell proliferation 2005 Feb;38(1):13-24
Cell proliferation 2005 Feb;38(1):13-24
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in HL60 cells were treated with camptothecin for 24 hours.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: cleaved Retinoblastoma 1 Antibody (172C1094) [NB100-56598] - Analysis in sections of primary human breast tumors. Image courtesy of Dr. Macleod, The Ben May Institute for Cancer Research, and The University of Chicago.
