Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- LS-B34 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B34, RRID:AB_2248350
- Product name
- IHC-plus™ HDAC1 Antibody (aa450-482) LS-B34
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-HDAC-1 Antibody - Western Blot. Western blot of Affinity Purified anti-HDAC-1 antibody shows detection of a band at ~65 kD corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1350. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-HDAC-1 Antibody - Western Blot. HDAC1 Antibody was used for Western blot of LNCaP prostate cancer cells. Lysate was loaded at 50 ug per lane and incubated with 1:1000 dilution of primary Ab (Lot. 16248). A band was detected at the expected molecular weight of 55 Kda. Personal communication Flavio Rizzolio, Temple University.