H00117584-M01

antibody from Novus Biologicals

Targeting: RFFL CARP-2, CARP2, fring, rififylin, RNF189, RNF34L

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00117584-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00117584-M01, RRID:AB_2178609

Product name

Mouse Monoclonal Rffl Antibody

Antibody type

Monoclonal

Description

IgG purified. RFFL - rififylin

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Rffl Antibody (3A4) [H00117584-M01] - Analysis of RFFL over-expressed 293 cell line, cotransfected with RFFL Validated Chimera RNAi ( Cat # H00117584-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RFFL monoclonal antibody (M01), clone 3A4 (Cat # H00117584-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Rffl Antibody (3A4) [H00117584-M01] - Analysis of RFFL expression in transfected 293T cell line by RFFL monoclonal antibody (M01), clone 3A4.Lane 1: RFFL transfected lysate(36.6 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: Rffl Antibody (3A4) [H00117584-M01] - Detection limit for recombinant GST tagged RFFL is 0.03 ng/ml as a capture antibody.