Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB10075 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10075, RRID:AB_1676483
- Product name
- MAD2L2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of MAD2L2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references MAD2B is an inhibitor of the anaphase-promoting complex.
A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2.
Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta.
Chen J, Fang G
Genes & development 2001 Jul 15;15(14):1765-70
Genes & development 2001 Jul 15;15(14):1765-70
A human REV7 homolog that interacts with the polymerase zeta catalytic subunit hREV3 and the spindle assembly checkpoint protein hMAD2.
Murakumo Y, Roth T, Ishii H, Rasio D, Numata S, Croce CM, Fishel R
The Journal of biological chemistry 2000 Feb 11;275(6):4391-7
The Journal of biological chemistry 2000 Feb 11;275(6):4391-7
Evidence for an interaction of the metalloprotease-disintegrin tumour necrosis factor alpha convertase (TACE) with mitotic arrest deficient 2 (MAD2), and of the metalloprotease-disintegrin MDC9 with a novel MAD2-related protein, MAD2beta.
Nelson KK, Schlöndorff J, Blobel CP
The Biochemical journal 1999 Nov 1;343 Pt 3:673-80
The Biochemical journal 1999 Nov 1;343 Pt 3:673-80
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblotting of MAD2L2 polyclonal antibody (Cat # PAB10075) was used at a 1:500 dilution to detect human MAD2L2 by western blot.Both HeLa whole cell lysate (Lane 1) and nuclear lysate (Lane 2) were probed using this antibody.This antibody clearly detects a ~20 KDa band corresponding to human MAD2L2 (predicted molecular weight is 24 KDa).Approximately 20 ug of each lysate was loaded on a 10% SDS-PAGE.Primary antibody was reacted with the membrane at room temperature for 1 h.After subsequent washing, a 1:2,000 dilution of HRP conjugated Gt-a-Rabbit IgG was used for visualization. Exposure time was 30 sec.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry of MAD2L2 polyclonal antibody (Cat # PAB10075) shows strong nuclear and cytoplasmic staining of tumor cells in cancerous human kidney tissue.Tissue was formalin-fixed and paraffin embedded.Brown color indicates presence of protein, blue color shows cell nuclei.Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)