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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- RQ8290 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MAD2L2 Antibody / MAD2B / REV7
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 ug
- Storage
- After reconstitution, the MAD2B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A375, 2) 293T, 3) HeLa, 4) K562, 5) Jurkat, 6) ThP-1 and 7) SH-SY5Y cell lysate with MAD2B antibody. Predicted molecular weight ~24 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with MAD2B antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer tissue with MAD2B antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human HeLa cells with MAD2B antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MAD2B antibody.
