Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-66728 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SFRP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LKSEAIIEHLC ASEFALRMKI KEVKKENGDK KIVPKKKKPL KLGPIKKKDL
- Concentration
- 0.05 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SFRP1 in human cell line RT-4. Samples were probed using a SFRP1 Polyclonal Antibody (Product # PA5-66728).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot was performed using Anti-SFRP1 Polyclonal Antibody (Product # PA5-66728) and a 35 kDa band corresponding to SFRP1 was observed across cell lines and tissue extract tested in comparison to MCF-7, SK-OV-3 and U-937 which is reported to be low. An uncharacterised band (*) at ~45 kDA was also observed. Whole cell extracts (30 µg lysate) of MCF 10A (Lane 1), MCF 10A treated with PTI (1X for 4hr) (Lane 2), MCF7 (Lane 3), MCF7 treated with PTI (1X for 4hr) (Lane 4), NIH:OVCAR-3 (Lane 5), SK-O-V3 (Lane 6), U-937 (Lane 7) and tissue extract of Mouse Kidney (Lane 8) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of SFRP1 in human cell line SK-MEL-30 shows localization to nucleoli. Samples were probed using a SFRP1 Polyclonal Antibody (Product # PA5-66728).