PA5-119647

antibody from Invitrogen Antibodies

Targeting: SFRP1 FRP, FRP-1, SARP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-119647

Provider

Invitrogen Antibodies

Product name

SFRP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Control: A549, SH-SY-5Y, rat testis tissue, human colon cancer tissue, human breast tissue, human placenta tissue, mouse small intestine tissue. Subcellular Location: Secreted.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SFRP1 on SH-SY-5Y cells lysates using SFRP1 Polyclonal Antibody (Product # PA5-119647).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SFRP1 in SH-SY-5Y cells (green) using SFRP1 Polyclonal Antibody (Product # PA5-119647). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SFRP1 in A549 cells (green) using SFRP1 Polyclonal Antibody (Product # PA5-119647). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat testis tissue using SFRP1 Polyclonal Antibody (Product # PA5-119647). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human colon cancer tissue using SFRP1 Polyclonal Antibody (Product # PA5-119647). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human breast tissue using SFRP1 Polyclonal Antibody (Product # PA5-119647). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human placenta tissue using SFRP1 Polyclonal Antibody (Product # PA5-119647). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse small intestine tissue using SFRP1 Polyclonal Antibody (Product # PA5-119647). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of SFRP1 in SH-SY-5Y cells using SFRP1 Polyclonal Antibody (Product # PA5-119647) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.