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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NB600-499 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-499, RRID:AB_2186947
- Product name
- Rabbit Polyclonal sFRP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. SFRP1
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
aa 42-53- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: sFRP-1 Antibody [NB600-499] - Analysis of a band 37 kDa (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4C. Signal was detected using standard techniques. Personnel communication Becky Mercer, University of Columbia.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: sFRP-1 Antibody [NB600-499] - Used at a 1:800 dilution for 20 min to detect SFRP in human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG is suitable for secondary antibody detection.
