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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP09234PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09234PU-N, RRID:AB_2035953
- Product name
- anti SFRP1
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to a 12 amino acid region of Human Sfrp1 protein
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using SFRP1 Antibody Cat.-No AP09234PU-N shows detection of a band ~37 kDa band (arrowhead) corresponding to SFRP1 in lysates from human cultured airway epithelial cells. Lysates were run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:230 dilution of anti-SFRP1 antibody overnight at 4°C. Signal was detected using standard techniques. Personnel communication Becky Mercer, University of Columbia.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- SFRP1 Antibody Cat.-No AP09234PU-N was used at a 1/800 dilution for 20 min to detect SFRP in Human dermal hypertrophic scar tissue. Tissue was formalin-fixed followed by heat mediated antigen retrieval prior to blocking. HRP Gt-a-Rabbit IgG is suitable for secondary antibody detection.
