V2218

antibody from NSJ Bioreagents

Targeting: PCNA

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V2218

Provider

NSJ Bioreagents

Product name

PCNA Antibody

Antibody type

Monoclonal

Antigen

Rat PCNA / Protein A fusion protein was used as the immunogen for this antibody.

Description

Protein G purified antibody

Reactivity

Human, Mouse, Rat, Chicken/Avian, Drosophila, Porcine, Simian, Zebrafish

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

PC10

Vial size

20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)

Storage

Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

References

Submitted references

Darbepoetin-α accelerates neovascularization and engraftment of extrahepatic colorectal metastases.

Senger S, Kollmar O, Menger MD, Schilling MK, Rupertus K
European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes 2014;53(1-4):25-36

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DDB2 association with PCNA is required for its degradation after UV-induced DNA damage.

Cazzalini O, Perucca P, Mocchi R, Sommatis S, Prosperi E, Stivala LA
Cell cycle (Georgetown, Tex.) 2014;13(2):240-8

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Altered expression of p21, activated caspase-3, and PCNA in bronchiolar epithelium of smokers with and without chronic obstructive pulmonary disease.

Chiappara G, Gjomarkaj M, Sciarrino S, Vitulo P, Pipitone L, Pace E
Experimental lung research 2014 Sep;40(7):343-53

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Histopathological lesions, P-glycoprotein and PCNA expression in zebrafish (Danio rerio) liver after a single exposure to diethylnitrosamine.

Machado SP, Cunha V, Reis-Henriques MA, Ferreira M
Environmental toxicology and pharmacology 2014 Nov;38(3):720-32

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Hypomorphic PCNA mutation underlies a human DNA repair disorder.

Baple EL, Chambers H, Cross HE, Fawcett H, Nakazawa Y, Chioza BA, Harlalka GV, Mansour S, Sreekantan-Nair A, Patton MA, Muggenthaler M, Rich P, Wagner K, Coblentz R, Stein CK, Last JI, Taylor AM, Jackson AP, Ogi T, Lehmann AR, Green CM, Crosby AH
The Journal of clinical investigation 2014 Jul;124(7):3137-46

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Expression of PCNA, p53 and Ki-67 in dentinogenic ghost cell tumors and calcifying cystic odontogenic tumors

Pulino B, Siqueira C, Duarte C, Sousa S
Clinical and Laboratorial Research in Dentistry 2014 February;20(1):16

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Spontaneous nephroblastoma with lung metastasis in a rat.

Ito Y, Matsushita K, Tsuchiya T, Kohara Y, Yoshikawa T, Sato M, Kitaura K, Matsumoto S
Journal of toxicologic pathology 2014 Apr;27(1):91-5

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Enhanced follicular recruitment and atresia in cortex derived from ovaries with endometriomas.

Kitajima M, Dolmans MM, Donnez O, Masuzaki H, Soares M, Donnez J
Fertility and sterility 2014 Apr;101(4):1031-7

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of HeLa cell lysate with PCNA antibody (clone PC10).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of human human tonsil stained with PCNA antibody (PC10).

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free PCNA Antibody (clone PC10). Confirmation of Integrity and Purity of the Antibody.