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LearnNB500-106
antibody from Novus Biologicals
Targeting: PCNA
Western blot
ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [32]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- NB500-106 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-106, RRID:AB_2252058
- Product name
- Mouse Monoclonal PCNA Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Specific for PCNA p36 protein expressed at high levels in proliferating cells.
- Reactivity
- Human, Mouse, Rat, Chicken/Avian, Drosophila, Porcine, Rabbit, Simian, Yeast, Zebrafish
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Histological differentiation of mucus cell subtypes suggests functional compartmentation in the eel esophagus.
Modulating EGFR-MTORC1-autophagy as a potential therapy for persistent fetal vasculature (PFV) disease.
OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary.
Morphological characterization of small hepatocytes after bile duct ligation in chicken.
Radical change of apoptotic strategy following irradiation during later period of embryogenesis in medaka (Oryzias latipes).
Neural Progenitors in the Developing Neocortex of the Northern Tree Shrew (Tupaia belangeri) Show a Closer Relationship to Gyrencephalic Primates Than to Lissencephalic Rodents.
Impairment of Host Liver Repopulation by Transplanted Hepatocytes in Aged Rats and the Release by Short-Term Growth Hormone Treatment.
Killing two birds with one stone: dual blockade of integrin and FGF signaling through targeting syndecan-4 in postoperative capsular opacification.
Chemerin Stimulates Vascular Smooth Muscle Cell Proliferation and Carotid Neointimal Hyperplasia by Activating Mitogen-Activated Protein Kinase Signaling.
Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt.
Regulation and function of SIRT1 in rheumatoid arthritis synovial fibroblasts.
Cell proliferation and apoptosis in optic nerve and brain integration centers of adult trout Oncorhynchus mykiss after optic nerve injury.
Synergy of TLR3 and 7 ligands significantly enhances function of DCs to present inactivated PRRSV antigen through TRIF/MyD88-NF-κB signaling pathway.
Toxicity of pristine graphene in experiments in a chicken embryo model.
The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation.
Lactobacillus salivarius Ren prevent the early colorectal carcinogenesis in 1, 2-dimethylhydrazine-induced rat model.
ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability.
Demethoxycurcumin, a major active curcuminoid from Curcuma longa, suppresses balloon injury induced vascular smooth muscle cell migration and neointima formation: an in vitro and in vivo study.
Methoxyamine sensitizes the resistant glioblastoma T98G cell line to the alkylating agent temozolomide.
Lactobacillus salivarius REN inhibits rat oral cancer induced by 4-nitroquioline 1-oxide.
Hispolon attenuates balloon-injured neointimal formation and modulates vascular smooth muscle cell migration via AKT and ERK phosphorylation.
Imatinib mesylate improves liver regeneration and attenuates liver fibrogenesis in CCL4-treated mice.
Efficacy of Tie2 receptor antagonism in angiosarcoma.
Efficacy of Tie2 receptor antagonism in angiosarcoma.
Functional and physical interaction between the mismatch repair and FA-BRCA pathways.
Morphogenesis of the medaka cerebellum, with special reference to the mesencephalic sheet, a structure homologous to the rostrolateral part of mammalian anterior medullary velum.
Molecular mechanism of green microalgae, Dunaliella salina, involved in attenuating balloon injury-induced neointimal formation.
Cell proliferation dynamics of somatic and germline tissues during zooidal life span in the colonial tunicate Botryllus primigenus.
The assessment of proliferating cell nuclear antigen (PCNA)immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis.
The assessment of proliferating cell nuclear antigen (PCNA)immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis.
Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
Wong MK, Uchida M, Tsukada T
Cell and tissue research 2020 Jun;380(3):499-512
Cell and tissue research 2020 Jun;380(3):499-512
Modulating EGFR-MTORC1-autophagy as a potential therapy for persistent fetal vasculature (PFV) disease.
Yazdankhah M, Shang P, Ghosh S, Bhutto IA, Stepicheva N, Grebe R, Hose S, Weiss J, Luo T, Mishra S, Riazuddin SA, Ghosh A, Handa JT, Lutty GA, Zigler JS Jr, Sinha D
Autophagy 2020 Jun;16(6):1130-1142
Autophagy 2020 Jun;16(6):1130-1142
OTULIN Prevents Liver Inflammation and Hepatocellular Carcinoma by Inhibiting FADD- and RIPK1 Kinase-Mediated Hepatocyte Apoptosis.
Verboom L, Martens A, Priem D, Hoste E, Sze M, Vikkula H, Van Hove L, Voet S, Roels J, Maelfait J, Bongiovanni L, de Bruin A, Scott CL, Saeys Y, Pasparakis M, Bertrand MJM, van Loo G
Cell reports 2020 Feb 18;30(7):2237-2247.e6
Cell reports 2020 Feb 18;30(7):2237-2247.e6
SIRT1 participates in the response to methylglyoxal-dependent glycative stress in mouse oocytes and ovary.
Di Emidio G, Santini SJ, D'Alessandro AM, Vetuschi A, Sferra R, Artini PG, Carta G, Falone S, Amicarelli F, Tatone C
Biochimica et biophysica acta. Molecular basis of disease 2019 Jun 1;1865(6):1389-1401
Biochimica et biophysica acta. Molecular basis of disease 2019 Jun 1;1865(6):1389-1401
Morphological characterization of small hepatocytes after bile duct ligation in chicken.
Fukasawa H, Hashimoto H, Hiramoto K, Sugiyama M, Taniguchi K, Yoshioka K
Poultry science 2019 Feb 1;98(2):717-721
Poultry science 2019 Feb 1;98(2):717-721
Radical change of apoptotic strategy following irradiation during later period of embryogenesis in medaka (Oryzias latipes).
Yasuda T, Ishikawa Y, Shioya N, Itoh K, Kamahori M, Nagata K, Takano Y, Mitani H, Oda S
PloS one 2018;13(8):e0201790
PloS one 2018;13(8):e0201790
Neural Progenitors in the Developing Neocortex of the Northern Tree Shrew (Tupaia belangeri) Show a Closer Relationship to Gyrencephalic Primates Than to Lissencephalic Rodents.
Römer S, Bender H, Knabe W, Zimmermann E, Rübsamen R, Seeger J, Fietz SA
Frontiers in neuroanatomy 2018;12:29
Frontiers in neuroanatomy 2018;12:29
Impairment of Host Liver Repopulation by Transplanted Hepatocytes in Aged Rats and the Release by Short-Term Growth Hormone Treatment.
Stock P, Bielohuby M, Staege MS, Hsu MJ, Bidlingmaier M, Christ B
The American journal of pathology 2017 Mar;187(3):553-569
The American journal of pathology 2017 Mar;187(3):553-569
Killing two birds with one stone: dual blockade of integrin and FGF signaling through targeting syndecan-4 in postoperative capsular opacification.
Qin Y, Zhu Y, Luo F, Chen C, Chen X, Wu M
Cell death & disease 2017 Jul 13;8(7):e2920
Cell death & disease 2017 Jul 13;8(7):e2920
Chemerin Stimulates Vascular Smooth Muscle Cell Proliferation and Carotid Neointimal Hyperplasia by Activating Mitogen-Activated Protein Kinase Signaling.
Xiong W, Luo Y, Wu L, Liu F, Liu H, Li J, Liao B, Dong S
PloS one 2016;11(10):e0165305
PloS one 2016;11(10):e0165305
Polymerases ε and ∂ repair dysfunctional telomeres facilitated by salt.
Ivanova IG, Maringele L
Nucleic acids research 2016 May 5;44(8):3728-38
Nucleic acids research 2016 May 5;44(8):3728-38
Regulation and function of SIRT1 in rheumatoid arthritis synovial fibroblasts.
Engler A, Tange C, Frank-Bertoncelj M, Gay RE, Gay S, Ospelt C
Journal of molecular medicine (Berlin, Germany) 2016 Feb;94(2):173-82
Journal of molecular medicine (Berlin, Germany) 2016 Feb;94(2):173-82
Cell proliferation and apoptosis in optic nerve and brain integration centers of adult trout Oncorhynchus mykiss after optic nerve injury.
Pushchina EV, Shukla S, Varaksin AA, Obukhov DK
Neural regeneration research 2016 Apr;11(4):578-90
Neural regeneration research 2016 Apr;11(4):578-90
Synergy of TLR3 and 7 ligands significantly enhances function of DCs to present inactivated PRRSV antigen through TRIF/MyD88-NF-κB signaling pathway.
Hu Y, Cong X, Chen L, Qi J, Wu X, Zhou M, Yoo D, Li F, Sun W, Wu J, Zhao X, Chen Z, Yu J, Du Y, Wang J
Scientific reports 2016 Apr 5;6:23977
Scientific reports 2016 Apr 5;6:23977
Toxicity of pristine graphene in experiments in a chicken embryo model.
Sawosz E, Jaworski S, Kutwin M, Hotowy A, Wierzbicki M, Grodzik M, Kurantowicz N, Strojny B, Lipińska L, Chwalibog A
International journal of nanomedicine 2014;9:3913-22
International journal of nanomedicine 2014;9:3913-22
The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation.
Houlihan SL, Feng Y
eLife 2014 Sep 23;3:e03297
eLife 2014 Sep 23;3:e03297
Lactobacillus salivarius Ren prevent the early colorectal carcinogenesis in 1, 2-dimethylhydrazine-induced rat model.
Zhu J, Zhu C, Ge S, Zhang M, Jiang L, Cui J, Ren F
Journal of applied microbiology 2014 Jul;117(1):208-16
Journal of applied microbiology 2014 Jul;117(1):208-16
ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability.
Errami Y, Brim H, Oumouna-Benachour K, Oumouna M, Naura AS, Kim H, Ju J, Davis CJ, Kim JG, Ashktorab H, Fallon K, Xu M, Zhang J, Del Valle L, Boulares AH
PloS one 2013;8(2):e57871
PloS one 2013;8(2):e57871
Demethoxycurcumin, a major active curcuminoid from Curcuma longa, suppresses balloon injury induced vascular smooth muscle cell migration and neointima formation: an in vitro and in vivo study.
Sheu MJ, Lin HY, Yang YH, Chou CJ, Chien YC, Wu TS, Wu CH
Molecular nutrition & food research 2013 Sep;57(9):1586-97
Molecular nutrition & food research 2013 Sep;57(9):1586-97
Methoxyamine sensitizes the resistant glioblastoma T98G cell line to the alkylating agent temozolomide.
Montaldi AP, Sakamoto-Hojo ET
Clinical and experimental medicine 2013 Nov;13(4):279-88
Clinical and experimental medicine 2013 Nov;13(4):279-88
Lactobacillus salivarius REN inhibits rat oral cancer induced by 4-nitroquioline 1-oxide.
Zhang M, Wang F, Jiang L, Liu R, Zhang L, Lei X, Li J, Jiang J, Guo H, Fang B, Zhao L, Ren F
Cancer prevention research (Philadelphia, Pa.) 2013 Jul;6(7):686-94
Cancer prevention research (Philadelphia, Pa.) 2013 Jul;6(7):686-94
Hispolon attenuates balloon-injured neointimal formation and modulates vascular smooth muscle cell migration via AKT and ERK phosphorylation.
Chien YC, Huang GJ, Cheng HC, Wu CH, Sheu MJ
Journal of natural products 2012 Sep 28;75(9):1524-33
Journal of natural products 2012 Sep 28;75(9):1524-33
Imatinib mesylate improves liver regeneration and attenuates liver fibrogenesis in CCL4-treated mice.
Kuo WL, Yu MC, Lee JF, Tsai CN, Chen TC, Chen MF
Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract 2012 Feb;16(2):361-9
Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract 2012 Feb;16(2):361-9
Efficacy of Tie2 receptor antagonism in angiosarcoma.
Hasenstein JR, Kasmerchak K, Buehler D, Hafez GR, Cleary K, Moody JS, Kozak KR
Neoplasia (New York, N.Y.) 2012 Feb;14(2):131-40
Neoplasia (New York, N.Y.) 2012 Feb;14(2):131-40
Efficacy of Tie2 receptor antagonism in angiosarcoma.
Hasenstein JR, Kasmerchak K, Buehler D, Hafez GR, Cleary K, Moody JS, Kozak KR
Neoplasia (New York, N.Y.) 2012 Feb;14(2):131-40
Neoplasia (New York, N.Y.) 2012 Feb;14(2):131-40
Functional and physical interaction between the mismatch repair and FA-BRCA pathways.
Williams SA, Wilson JB, Clark AP, Mitson-Salazar A, Tomashevski A, Ananth S, Glazer PM, Semmes OJ, Bale AE, Jones NJ, Kupfer GM
Human molecular genetics 2011 Nov 15;20(22):4395-410
Human molecular genetics 2011 Nov 15;20(22):4395-410
Morphogenesis of the medaka cerebellum, with special reference to the mesencephalic sheet, a structure homologous to the rostrolateral part of mammalian anterior medullary velum.
Ishikawa Y, Yamamoto N, Yasuda T, Yoshimoto M, Ito H
Brain, behavior and evolution 2010;75(2):88-103
Brain, behavior and evolution 2010;75(2):88-103
Molecular mechanism of green microalgae, Dunaliella salina, involved in attenuating balloon injury-induced neointimal formation.
Sheu MJ, Cheng HC, Chien YC, Chou PY, Huang GJ, Chen JS, Lin SY, Wu CH
The British journal of nutrition 2010 Aug;104(3):326-35
The British journal of nutrition 2010 Aug;104(3):326-35
Cell proliferation dynamics of somatic and germline tissues during zooidal life span in the colonial tunicate Botryllus primigenus.
Kawamura K, Tachibana M, Sunanaga T
Developmental dynamics : an official publication of the American Association of Anatomists 2008 Jul;237(7):1812-25
Developmental dynamics : an official publication of the American Association of Anatomists 2008 Jul;237(7):1812-25
The assessment of proliferating cell nuclear antigen (PCNA)immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis.
Woods AL, Hall PA, Shepherd NA, Hanby AM, Waseem NH, Lane DP, Levison DA
Histopathology 2002 Sep;41(3A):165-71
Histopathology 2002 Sep;41(3A):165-71
The assessment of proliferating cell nuclear antigen (PCNA)immunostaining in primary gastrointestinal lymphomas and its relationship to histological grade, S + G2 + M phase fraction (flow cytometric analysis) and prognosis.
Woods AL, Hall PA, Shepherd NA, Hanby AM, Waseem NH, Lane DP, Levison DA
Histopathology 2002 Sep;41(3A):165-71
Histopathology 2002 Sep;41(3A):165-71
Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
Waseem NH, Lane DP
Journal of cell science 1990 May;96 ( Pt 1):121-9
Journal of cell science 1990 May;96 ( Pt 1):121-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody (PC10) [NB500-106] - HeLa cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody (PC10) [NB500-106] - Analysis of human (HeLa lysate), murine (SV-T2 lysate), bovine (BAEC lysate), and porcine (PAE lysate) cell extracts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PCNA Antibody (PC10) [NB500-106] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/mL anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: PCNA Antibody (PC10) [NB500-106] - Paraformaldehyde fixed frozen section of brain from murine embryo using PCNA antibody clone PC10 (green), an IB4 antibody (red) and DAPI. Image provided by Dr. Siegenthaler via product review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Analysis of PCNA in mouse cornea. Image courtesy of product review by Bo-Yie Chen.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PCNA Antibody (PC10) [NB500-106] - An intracellular stain was performed on HepG2 cells with PCNA Antibody (PC10) NB500-106G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 488.
