H00005111-M04

antibody from Abnova Corporation

Targeting: PCNA

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005111-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005111-M04, RRID:AB_566066

Product name

PCNA monoclonal antibody (M04), clone S1

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant PCNA.

Antigen sequence

MFEARLVQGSILKKVLEALKDLINEACWDISSSGV
NLQSMDSSHVSLVQLTLRSEGFDTYRCDRNLAMGV
NLTSMSKILKCAGNEDIITLRAEDNADTLALVFEA
PNQEKVSDYEMKLMDLDVEQLGIPEQEYSCVVKMP
SGEFARICRDLSHIGDAVVISCAKDGVKFSASGEL
GNGNIKLSQTSNVDKEEEAVTIEMNEPVQLTFALR
YLNFFTKATPLSSTVTLSMSADVPLVVEYKIADMG
HLKYYLAPKIEDEEGS


Isotype

IgG

Antibody clone number

S1

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

DNA topoisomerase III alpha regulates p53-mediated tumor suppression.

Hsieh MY, Fan JR, Chang HW, Chen HC, Shen TL, Teng SC, Yeh YH, Li TK
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Mar 15;20(6):1489-501

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

PCNA monoclonal antibody (M04), clone S1. Western Blot analysis of PCNA expression in Raw 264.7 ( Cat # L024V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody (M04), clone S1.Lane 1: PCNA transfected lysate(28.8 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged PCNA is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol